지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
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Mesenchymal stem cells (MSCs) have the ability to self-renew and differentiate into multi-lineage cells, thus highlighting the feasibility of using umbilical cord blood-derived MSCs (UCB-MSCs) for cell-therapy and tissueengineering. However, the low numbers of UCB-MSC derived from clinical samples requires that an ex vivo expansion step be implemented. As most stem cells reside in low oxygen tension environments (i.e., hypoxia), we cultured the UCBMSCs under 3% O<SUB>2</SUB> or 21% O<SUB>2</SUB> and the following parameters were examined: proliferation, senescence, differentiation and stem cell specific gene expression. UCB-MSCs cultured under hypoxic conditions expanded to significantly higher levels and showed less senescence compared to UCB-MSCs cultured under normoxic conditions. In regards to differentiation potential, UCB-MSCs cultured under hypoxic and normoxic conditions both underwent similar levels of osteogenesis as determined by ALP and von Kossa assay. Furthermore, UCB-MSCs cultured under hypoxic conditions exhibited higher expression of OCT4, NANOG and SOX2 genes. Moreover, cells expanded under hypoxia maintained a stem cell immnunophenotype as determined by flow cytometry. These results demonstrate that the expansion of human UCB-MSCs under a low oxygen tension microenvironment significantly improved cell proliferation and differentiation. These results demonstrate that hypoxic culture can be rapidly and easily implemented into the clinical-scale expansion process in order to maximize UCB-MSCs yield for application in clinical settings and at the same time reduce culture time while maintaining cell product quality.
목차
INTRODUCTION
MATERIAL AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
1. [학술지(정기간행물)] - Alves H / 2012 / A mesenchymal stromal cell gene signature for donor age / PloS One 7 : e42908 ~
2. [학술지(정기간행물)] - Avanzini MA / 2009 / Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors / Haematologica 94 : 1649 ~ 1660
3. [학술지(정기간행물)] - Babaie Y / 2007 / Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells / Stem Cells 25 : 500 ~ 510
4. [학술지(정기간행물)] - Braun RD / 2001 / Comparison of tumor and normal tissue oxygen tension measurements usingOxyLite or microelectrodes in rodents / Am J Physiol Heart Circ Physiol 280 : H2533 ~ 2544
5. [학술지(정기간행물)] - Caplan AI / 2007 / Adult mesenchymal stem cells for tissue engineering versus regenerative medicine / J Cell Physiol 213 : 341 ~ 347
2. [학술지(정기간행물)] - Avanzini MA / 2009 / Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors / Haematologica 94 : 1649 ~ 1660
3. [학술지(정기간행물)] - Babaie Y / 2007 / Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells / Stem Cells 25 : 500 ~ 510
4. [학술지(정기간행물)] - Braun RD / 2001 / Comparison of tumor and normal tissue oxygen tension measurements usingOxyLite or microelectrodes in rodents / Am J Physiol Heart Circ Physiol 280 : H2533 ~ 2544
5. [학술지(정기간행물)] - Caplan AI / 2007 / Adult mesenchymal stem cells for tissue engineering versus regenerative medicine / J Cell Physiol 213 : 341 ~ 347
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UCI(KEPA) : I410-ECN-0101-2016-510-001348001