MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver

Fang Tian; Hui-Min Ying; Yuan-Yuan Wang; Bo-Ning Cheng; Juan Chen

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자료유형: 학술저널

저자정보: Fang Tian (Xixi Hospital of Hangzhou Affiliated to Zhejiang Chinese Medical University) Hui-Min Ying (Xixi Hospital of Hangzhou Affiliated to Zhejiang Chinese Medical University) Yuan-Yuan Wang (Xixi Hospital of Hangzhou Affiliated to Zhejiang Chinese Medical University) Bo-Ning Cheng (Xixi Hospital of Hangzhou Affiliated to Zhejiang Chinese Medical University) Juan Chen (Xixi Hospital of Hangzhou Affiliated to Zhejiang Chinese Medical University)

저널정보: 연세대학교 의과대학 Yonsei Medical Journal Yonsei Medical Journal 제61권 제9호

발행연도: 2020.1

수록면: 780 - 788 (9page)

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Purpose: This research was designed to investigate how miR-542-5p regulates the progression of hyperglycemia and hyperlipoidemia. Materials and Methods: An in vivo model with diabetic db/db mice and an in vitro model with forskolin/dexamethasone (FSK/DEX)-induced primary hepatocytes and HepG2 cells were employed in the study. Bioinformatics analysis was conducted to identifythe expression of candidate miRNAs in the liver tissues of diabetic and control mice. H&E staining revealed liver morphologyin diabetic and control mice. Pyruvate tolerance tests, insulin tolerance tests, and intraperitoneal glucose tolerance test were utilizedto assess insulin resistance. ELISA was conducted to evaluate blood glucose and insulin levels. Red oil O staining showed lipiddeposition in liver tissues. Luciferase reporter assay was used to depict binding between miR-542-5p and forkhead box O1 (FOXO1). Results: MiR-542-5p expression was under-expressed in the livers of db/db mice. Further in vitro experiments revealed that FSK/DEX, which mimics the effects of glucagon and glucocorticoids, induced cellular glucose production in HepG2 cells and in primaryhepatocytes cells. Notably, these changes were reversed by miR-542-5p. We found that transcription factor FOXO1 is a target ofmiR-542-5p. Further in vivo study indicated that miR-542-5p overexpression decreases FOXO1 expression, thereby reversing increasesin blood glucose, blood lipids, and glucose-related enzymes in diabetic db/db mice. In contrast, anti-miR-542-5p exertedan adverse influence on blood glucose and blood lipid metabolism, and its stimulatory effects were significantly inhibited by sh-FOXO1 in normal control mice. Conclusion: Collectively, our results indicated that miR-542-5p inhibits hyperglycemia and hyperlipoidemia by targeting FOXO1.

#miR-542-5p #forkhead box O1 #diabetes #hyperglycemia #hyperlipoidemia

참고문헌 (32)

참고문헌 신청

Cheng YS / 2015 / PPP2R5C couples hepatic glucose and lipid homeostasis / PLoS Genet 11:e1005561

Bechmann LP / 2012 / The interaction of hepatic lipid and glucose metabolism in liver diseases / J Hepatol 56:952~964

Zhang C / 2019 / MicroRNA-338-3p suppresses cell proliferation, migration and invasion in human malignant melanoma by targeting MACC1 / Exp Ther Med 18:997~1004

Song Y / 2019 / Down-regulation of MicroRNA-592 in obesity contributes to hyperglycemia and insulin resistance / EBioMedicine 42:494~503

Esau C / 2006 / miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting / Cell Metab 3:87~98

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