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논문 기본 정보

자료유형
학술저널
저자정보
Anastasia Kubarova (부경대학교) 고혜진 (부경대학교) 박남규 (부경대학교)
저널정보
한국수산과학회 Fisheries and Aquatic Sciences Fisheries and Aquatic Sciences 제24권 제4호
발행연도
2021.1
수록면
163 - 170 (8page)

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초록· 키워드

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Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions, materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide, LC-MS and Edman degradation method were used, respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study, a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.
#Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions #materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide #LC-MS and Edman degradation method were used #respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study #a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.

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참고문헌 (31)

참고문헌 신청
Anderson JM / 1953 / Structure and function in the pyloric caeca of Asterias forbesi / Biol Bull Rev 105:47~61 google schola Anderson JM / 1959 / Studies on the cardiac stomach of a starfish, Patiria miniata(Brandt) / Biol Bull Rev 117:185~201 google schola Anderson JM / 1979 / Histological studies on the pyloric stomach and its appendages in Oreaster reticulatus (L.) (Asteroidea) / Biol Bull Rev 156:1~19 google schola Chia FS / 1969 / Histology of the pyloric caeca and its changes during brooding and starvation in a starfish, Leptasterias hexactis / Biol Bull Rev 136:185~192 google schola Davydov PV / 1990 / Animal species for developmental studies / Springer :287~311 google schola

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