메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류
인문학
인문학 일반 역사학 철학 종교학 어문학 기타 인문학
사회과학
사회과학 일반 경영학 관광학 교육학 군사학 문헌정보학 법학 사회학 신문방송학 심리과학 정치외교학 지리학 행정학 경제학 무역학 인류학 기타 사회과학
자연과학
자연과학 일반 물리학 생물학 생활과학 수학·통계학 천문학 지구과학 화학 기타 자연과학
공학
공학 일반 건축공학 기계공학 산업공학 재료·에너지공학 전기전자공학 조선해양공학 화학공학 컴퓨터학 기타공학
의약학
의학 일반 간호학 수의학 약학 한의학 기초의학 임상의학 기타 의약학
농수해양학
농수해양학 일반 농학 수산학 식품과학 임학 축산학 해상운송학 기타 농수해양학
예술체육학
예술 일반 디자인 미술 미용 사진 음악학 의상 체육 공연매체예술 기타 예술체육학
복합학
학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

ENG

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Chae Hyojin (Department of Laboratory Medicine College of Medicine The Catholic University of Korea Seoul KoreaC) Sung Pil Soo (Department of Internal Medicine Seoul St. Mary’s Hospital The Catholic University Liver Research Ce) Choi Hayoung (Catholic Genetic Laboratory Center Seoul St. Mary’s Hospital College of Medicine The Catholic Unive) Kwon Ahlm (Catholic Genetic Laboratory Center Seoul St. Mary’s Hospital College of Medicine The Catholic Unive) Kang Dain (Catholic Genetic Laboratory Center Seoul St. Mary’s Hospital College of Medicine The Catholic Unive) Kim Yonggoo (Department of Laboratory Medicine College of Medicine The Catholic University of Korea Seoul KoreaC) Kim Myungshin (Department of Laboratory Medicine College of Medicine The Catholic University of Korea Seoul KoreaC) Yoon Seung Kew (Department of Internal Medicine Seoul St. Mary’s Hospital The Catholic University Liver Research Ce)
저널정보
대한진단검사의학회 Annals of Laboratory Medicine Annals of Laboratory Medicine 제41권 제2호
발행연도
2021.1
수록면
198 - 206 (9page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Background: Hepatocellular carcinoma (HCC) is the second-most-common cause of cancer-related deaths worldwide, and an accurate and non-invasive biomarker for the early detection and monitoring of HCC is required. We assessed pathogenic variants of HCC driver genes in cell-free DNA (cfDNA) from HCC patients who had not undergone systemic therapy. Methods: Plasma cfDNA was collected from 20 HCC patients, and deep sequencing was performed using a customized cfDNA next-generation sequencing panel, targeting the major HCC driver genes (TP53, CTNNB1, TERT) that incorporates molecular barcoding. Results: In 13/20 (65%) patients, we identified at least one pathogenic variant of two major HCC driver genes (TP53 and CTNNB1), including 16 variants of TP53 and nine variants of CTNNB1. The TP53 and CTNNB1 variants showed low allele frequencies, with median values of 0.17% (range: 0.06%-6.99%) and 0.07% (range: 0.05%?0.96%), respectively. However, the molecular coverage of variants was sufficient, with median values of 5,543 (range: 2,317?9,088) and 7,568 (range: 2,400?9,633) for TP53 and CTNNB1 variants, respectively. Conclusions: Our targeted DNA sequencing successfully identified low-frequency pathogenic variants in the cfDNA from HCC patients by achieving high coverage of unique molecular families. Our results support the utility of cfDNA analysis to identify somatic gene variants in HCC patients.
#Hepatocellular carcinoma #Cell-free DNA #Next-generation sequencing #Molecular barcoding #Pathogenic variants #TP53 #CTNNB1 #TERT

목차

등록된 정보가 없습니다.

참고문헌 (25)

참고문헌 신청
Ferlay J / 2015 / Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012 / Int J Cancer 136:E359~E386 google schola Korean Liver Cancer Association (KLCA) and National Cancer Center (NCC / 2019 / 2018 Korean Liver Cancer Association–National Cancer Center Korea Practice Guidelines for the Management of Hepatocellular Carcinoma / Gut and Liver 13(3):227~299 Crossref Zhaleh Farokhizadeh / 2019 / Association of MicroRNA Polymorphisms With Hepatocellular Carcinoma in an Iranian Population / Annals of Laboratory Medicine 39(1):58~66 Crossref Ju Dong Yang / 2019 / Detect or not to detect very early stage hepatocellular carcinoma? The western perspective / Clinical and Molecular Hepatology 25(4):335~343 dbpia Yang K / 2019 / Pathologic complete response to chemoembolization improves survival outcomes after curative surgery for hepatocellular carcinoma: predictive factors of response / HPB (Oxford) 21:1718~1726 google schola

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0