지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 328 - 336 (9page)
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초록· 키워드
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ObjectiveCirculating cell-free tumor DNA (cfDNA) is the DNA released by apoptotic and necrotic cells of the primary tumor intothe blood during the period of tumor development. The cfDNA reflects the genetic and epigenetic alterations of theoriginal tumor. TP53 mutations are a defining feature of high-grade serous ovarian carcinoma. We optimized themethods for detecting TP53 mutations in cfDNA from blood samples. We confirmed the correlation of TP53 mutationin primary ovarian cancer tissue and it in cfDNA using digital polymerase chain reaction (dPCR). MethodsWe found 12 frequent mutation sites in TP53 using The Cancer Genome Atlas and Catalogue of Somatic Mutationsin Cancer data and manufactured 12 primers. The mutations in tissues were evaluated in fresh-frozen tissue (FFT) andformalin-fixed paraffin-embedded tissue (FFPET). We performed a prospective analysis of serial plasma samples collectedfrom 4 patients before debulking surgery. We extracted cfDNA and calculated its concentration in blood. dPCR was usedto analyze TP53 mutations in cfDNA, and we compared TP53 mutations in ovarian cancer tissue with those in cfDNA. ResultsTen primers out of 12 detected the presence of TP53 mutations in FFT, FFPET, and cfDNA. In FFT and FFPET tissue,there were no significant differences. The average cfDNA concentration was 2.12±0.59 ng/mL. We also confirmed thatmutations of cfDNA and those of FFT were all in R282W site. ConclusionThis study developed detection methods for TP53 mutations in cfDNA in ovarian cancer patients using dPCR. Theresults demonstrated that there are the same TP53 mutations in both ovarian cancer tissue and cfDNA.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Rivlin N / 2011 / Mutations in the p53 tumor suppressor gene: important milestones at the various steps of tumorigenesis / Genes Cancer 2:466~474
Cho Y / 1994 / Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations / Science 265:346~355
Mandel P / 1948 / Les acides nucleiques du plasma sanguin chez l'homme / C R Seances Soc Biol Fil 142:241~243
Sorenson GD / 1994 / Soluble normal and mutated DNA sequences from single-copy genes in human blood / Cancer Epidemiol Biomarkers Prev 3:67~71
Vasioukhin V / 1994 / Point mutations of the N-ras gene in the blood plasma DNA of patients with myelodysplastic syndrome or acute myelogenous leukaemia / Br J Haematol 86:774~779
Cho Y / 1994 / Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations / Science 265:346~355
Mandel P / 1948 / Les acides nucleiques du plasma sanguin chez l'homme / C R Seances Soc Biol Fil 142:241~243
Sorenson GD / 1994 / Soluble normal and mutated DNA sequences from single-copy genes in human blood / Cancer Epidemiol Biomarkers Prev 3:67~71
Vasioukhin V / 1994 / Point mutations of the N-ras gene in the blood plasma DNA of patients with myelodysplastic syndrome or acute myelogenous leukaemia / Br J Haematol 86:774~779
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