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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 480 - 490 (11page)
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초록· 키워드
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A novel lipase gene, Lip-1420, was isolated from a metagenomic library constructed from reed marsh from Mt. Jumbong in Korea, comprising 112,500 members of recombinant plasmids. The DNA sequence of Lip- 1420-subclone (5,513 bp) was found to contain at least 11 ORFs according to the GenBank database. The ORF-3 gene was inserted into the pET21a plasmid containing the C-terminal 6-His tag and transformed into E. coli BL21(DE3) to express the recombinant lipase protein. Lip-1420 was purified using a fast protein liquid chromatography system. The gene was registered in GenBank (MH628529). The values of Km and Vmax were determined as 0.268 mM and 1.821 units, respectively, at 40℃ and pH 8.0, using p-nitrophenyl palmitate as the substrate. This lipase belongs to family IV taxonomically because it has conserved HGGG and GDSAG motifs in the constitutive amino acid sequence. According to the predicted structural model, the binding sites are represented by residues H78, G81, D150, S151, A152, V181, and D236. Finally, Lip-1420 showed triolein selectivity for methanolysis between triolein (18:1) and tristearin (18:0) substrates. Further study of the selective mechanism and structure-function relationship of this new lipase could be useful for more practical applications.
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참고문헌 (37)
참고문헌 신청
Gupta R / 2004 / Bacterial lipases : an overview of production, purification and biochemical properties / Appl. Mocrobiol. Biotechnol 64:763~781
Kumar A / 2016 / Lipase catalysis in organic solvents : advantages and applications / Biol. Proced. Online 18:2
Bajaj A / 2010 / Biodiesel production through lipase catalyzed transesterification / J. Mol. Catal. B : Enzym 62:9~14
Torsvik V / 1990 / High diversity in DNA of soil bacteria / Appl. Environ. Microbiol 56:782~787
Steele HL / 2009 / Advances in recovery of novel biocatalysts from metagenomes / J. Mol. Microbiol. Biotechnol 16:25~37
Kumar A / 2016 / Lipase catalysis in organic solvents : advantages and applications / Biol. Proced. Online 18:2
Bajaj A / 2010 / Biodiesel production through lipase catalyzed transesterification / J. Mol. Catal. B : Enzym 62:9~14
Torsvik V / 1990 / High diversity in DNA of soil bacteria / Appl. Environ. Microbiol 56:782~787
Steele HL / 2009 / Advances in recovery of novel biocatalysts from metagenomes / J. Mol. Microbiol. Biotechnol 16:25~37
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