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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제6호
- 발행연도
- 2015.1
- 수록면
- 845 - 855 (11page)
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The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60oC and kept 45% enzyme activity after 1 h incubation at 70oC. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).
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참고문헌 (24)
참고문헌 신청
Anderson EM / 1998 / One biocatalystmany applications: the use of Candida antarctica B-lipase in organic synthesis / Biocatal. Biotransform 16 : 181 ~ 204
Bian CB / 2005 / Purification and preliminary crystallographic analysis of a Penicillium expansum lipase [J] / Biochim. Biophys. Acta 1752 : 99 ~ 102
Chang SW / 2006 / Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1
Chen JC / 1993 / Lipase production by Trichosporon fermentans WU-C12, a newly isolated yeast / J. Ferment. Bioeng 73 : 412 ~ 414
Chen SJ / 1998 / Production of an alkaline lipase by Acinetobacter radioresistens / J. Ferment. Bioeng 86 : 308 ~ 312
Bian CB / 2005 / Purification and preliminary crystallographic analysis of a Penicillium expansum lipase [J] / Biochim. Biophys. Acta 1752 : 99 ~ 102
Chang SW / 2006 / Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1
Chen JC / 1993 / Lipase production by Trichosporon fermentans WU-C12, a newly isolated yeast / J. Ferment. Bioeng 73 : 412 ~ 414
Chen SJ / 1998 / Production of an alkaline lipase by Acinetobacter radioresistens / J. Ferment. Bioeng 86 : 308 ~ 312
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