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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제5호
- 발행연도
- 2018.1
- 수록면
- 739 - 747 (9page)
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초록· 키워드
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The low solubility and high-cost recovery of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) are key problems in the bioproduction of high value-added conjugated linoleic acid (CLA). To improve the solubility of recombinant PAI, six chaperone proteins were coexpressed with PAI. Introduction of GroELS proteins dramatically improved the PAI solubility from 29% to 97%, with increased activity by 57.8%. Combined expression of DnaKJGrpE and GroELS proteins increased the activity by 11.9%. In contrast, coexpression of DnaKJGrpE proteins significantly reduced the activity by 57.4%. Plasmids pTf16 harboring the tig gene and pG-Tf2 containing the tig and groEL-groES genes had no visible impact on PAI expression. The lytic protein E was then introduced into the recombinant Escherichia coli to develop a cell autolysis system. A 35% activity of total intracellular PAI was released from the cytoplasm by suspending the lysed cells in distilled water. The PAI recovery was further improved to 81% by optimizing the release conditions. The lipase from Rhizopus oryzae was also expressed in E. coli, with an extracellular activity of 110.9 U/ml. By using the free PAI and lipase as catalysts, a joint system was established for producing CLA from sunflower oil. Under the optimized conditions, the maximum titer of t-10,c-12-CLA reached 9.4 g/l. This work provides an effective and low-cost strategy to improve the solubility and recovery of the recombinant intracellular PAI for further large-scale production of CLA.
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참고문헌 (31)
참고문헌 신청
Bhattacharya A / 2006 / Biological effects of conjugated linoleic acids in health and disease / J. Nutr. Biochem. 17:789~810
Watras AC / 2007 / The role of conjugated linoleic acid in reducing body fat and preventing holiday weight gain / Int. J. Obes. 31:481~487
Wang QY / 2013 / Conjugated linoleic acid production by alkali isomerization of linoleic acid from Idesia polycarpa Maxim. var. vestita Diels oil / Asian J. Chem. 25:3744~3748
Wahle KWJ / 2004 / Conjugated linoleic acids: are they beneficial or detrimental to health? / Prog. Lipid Res. 43:553~587
JM B / 2003 / Conjugated linoleic acid in humans: regulation of adiposity and insulin sensitivity / J. Nutr. 133:3041~3046
Watras AC / 2007 / The role of conjugated linoleic acid in reducing body fat and preventing holiday weight gain / Int. J. Obes. 31:481~487
Wang QY / 2013 / Conjugated linoleic acid production by alkali isomerization of linoleic acid from Idesia polycarpa Maxim. var. vestita Diels oil / Asian J. Chem. 25:3744~3748
Wahle KWJ / 2004 / Conjugated linoleic acids: are they beneficial or detrimental to health? / Prog. Lipid Res. 43:553~587
JM B / 2003 / Conjugated linoleic acid in humans: regulation of adiposity and insulin sensitivity / J. Nutr. 133:3041~3046
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