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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 207 - 213 (7page)
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In eukaryotic cells, organelles are distributed and positioned in proximity to each other through molecular tether proteins. Among these, the mitochondria-endoplasmic reticulum cortex anchor (MECA) is a well-known tethering complex in Saccharomyces cerevisiae that tethers mitochondria to the plasma membrane and plays a key role in mitochondrial fission. The main components of MECA are Num1 and Mdm36, and it is known that Mdm36 binds to Num1 to enhance mitochondrial tethering. To better understand the biochemical characteristics of the Num1-Mdm36 complex at the molecular level, we purified the coiled-coil domain of Num1, full-length Mdm36, and Num1-Mdm36 complex and identified the oligomeric state and stoichiometric characteristics of the Num1-Mdm36 complex by chemical crosslinking, size-exclusion chromatography coupled with multi-angle light scattering, and isothermal titration calorimetry. Mdm36 exists as a dimer and interacts preferentially with Num1 with a stoichiometry of 2:2, forming a heterotetrameric complex. Furthermore, we narrowed down the specific binding region of Num1, which is essential for interacting with Mdm36, and showed that their binding affinity is strong enough to tether both mitochondrial and plasma membranes. Our biochemical characterizations suggest a stoichiometric model of the Num1-Mdm36 complex at the mitochondria-plasma membrane contact site in budding yeast.
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참고문헌 (26)
참고문헌 신청
Booth, D. M. / 2016 / Redox nanodomains are induced by and control calcium signaling at the ER-mitochondrial interface / Mol. Cell 63:240~248
Cerveny, K. L. / 2007 / Yeast mitochondrial division and distribution require the cortical num1 protein / Dev. Cell 12:363~375
Chen, W. / 2018 / Direct membrane binding and self-interaction contribute to Mmr1 function in mitochondrial inheritance / Mol. Biol. Cell 29:2346~2357
de Brito, O. M. / 2008 / Mitofusin 2 tethers endoplasmic reticulum to mitochondria / Nature 456:605~610
Dong, D. / 2020 / Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome / Light Sci. Appl 9:11
Cerveny, K. L. / 2007 / Yeast mitochondrial division and distribution require the cortical num1 protein / Dev. Cell 12:363~375
Chen, W. / 2018 / Direct membrane binding and self-interaction contribute to Mmr1 function in mitochondrial inheritance / Mol. Biol. Cell 29:2346~2357
de Brito, O. M. / 2008 / Mitofusin 2 tethers endoplasmic reticulum to mitochondria / Nature 456:605~610
Dong, D. / 2020 / Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome / Light Sci. Appl 9:11
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