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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 337 - 344 (8page)
- DOI
- 10.7783/KJMCS.2021.29.5.337
이용수
초록· 키워드
오류제보하기
Background: The root and bark of Cercis chinensis Bunge have been used as Chinese herbal medicines for stroke, hypertension, and gynecological diseases. The seed of C. chinensis is classified as a food ingredient in the Food Public Code. In this study, the inhibitory activities of Cercis chinensis Bunge seed against beta-amyloid (Aβ) secretion and acetylcholinesterase (AChE) were investigated.
Methods and Results: The effect of 50% ethanolic extract of C. chinensis Bunge seed (CCS) on Aβ secretion in APPswe cells was analyzed by Aβ enzyme-linked immunosorbent assay (ELISA). CCS dose-dependently decreased Aβ1-42 secretion at 50 and 100 ㎍/㎖. The half-maximal inhibitory concentration (IC50) values of CCS were 45.02 ㎍/㎖ in Aβ1-40 and 43.48 ㎍/㎖ in Aβ1-42. To analyze the metabolism of amyloid precursor protein (APP) via β-secretase, the beta-carboxyl-terminal fragment (β-CTF) level was measured via ELISA and was found to be dose-dependently increased upon CSS treatment. Additionally, western blot analysis was performed to check the protein expression level of APP, soluble alpha (sAPPα), and CTF. The APP protein level did not change upon CCS treatment but sAPPα secretion level increased, indicating a tendency to increase both α- and β-CTF. These results suggest that CCS reduces Aβ secretion by targeting γ-secretase. Additionally, CCS showed a strong AChE inhibitory activity at an IC50 value of 8.37 ㎍/㎖.
Conclusions: CCS exhibited Aβ reduction via γ-secretase and AChE inhibition and may be used as a material to develop healthy functional foods and medicine for Alzheimer"s disease treatment.
Methods and Results: The effect of 50% ethanolic extract of C. chinensis Bunge seed (CCS) on Aβ secretion in APPswe cells was analyzed by Aβ enzyme-linked immunosorbent assay (ELISA). CCS dose-dependently decreased Aβ1-42 secretion at 50 and 100 ㎍/㎖. The half-maximal inhibitory concentration (IC50) values of CCS were 45.02 ㎍/㎖ in Aβ1-40 and 43.48 ㎍/㎖ in Aβ1-42. To analyze the metabolism of amyloid precursor protein (APP) via β-secretase, the beta-carboxyl-terminal fragment (β-CTF) level was measured via ELISA and was found to be dose-dependently increased upon CSS treatment. Additionally, western blot analysis was performed to check the protein expression level of APP, soluble alpha (sAPPα), and CTF. The APP protein level did not change upon CCS treatment but sAPPα secretion level increased, indicating a tendency to increase both α- and β-CTF. These results suggest that CCS reduces Aβ secretion by targeting γ-secretase. Additionally, CCS showed a strong AChE inhibitory activity at an IC50 value of 8.37 ㎍/㎖.
Conclusions: CCS exhibited Aβ reduction via γ-secretase and AChE inhibition and may be used as a material to develop healthy functional foods and medicine for Alzheimer"s disease treatment.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (31)
참고문헌 신청
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Cuppus AMW / 2012 / Plasma β amyloid and the risk of Alzheimer’s disease in Down syndrome / Neirobiology of Aging 33:1988~1994
Epis R / 2012 / Alpha, beta-and gamma-secretase in Alzheimer’s disease / Frontiers in Bioscience 4:1126~1150
엄현섭 / 2008 / 지구성 운동이 NSE/APPsw 알츠하이머 질환 생쥐의 인지능력, Aβ-42, BDNF, GLUT-1과 HSP-70 단백질 발현에 미치는 영향 / 생명과학회지 18(6):796~803
한설희 / 2009 / 알츠하이머병의 새로운 약물치료 / 대한의사협회지 52(11):1059~1068
Cuppus AMW / 2012 / Plasma β amyloid and the risk of Alzheimer’s disease in Down syndrome / Neirobiology of Aging 33:1988~1994
Epis R / 2012 / Alpha, beta-and gamma-secretase in Alzheimer’s disease / Frontiers in Bioscience 4:1126~1150
엄현섭 / 2008 / 지구성 운동이 NSE/APPsw 알츠하이머 질환 생쥐의 인지능력, Aβ-42, BDNF, GLUT-1과 HSP-70 단백질 발현에 미치는 영향 / 생명과학회지 18(6):796~803
한설희 / 2009 / 알츠하이머병의 새로운 약물치료 / 대한의사협회지 52(11):1059~1068
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