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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.2
- 수록면
- 157 - 166 (10page)
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초록· 키워드
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Coronavirus causes an infectious disease in various species and crosses the species barriers leading to the outbreak of zoonotic diseases. Due to the respiratory diseases are mainly caused in humans and viruses are replicated and excreted through the respiratory tract, the nasal fluid and sputum are mainly used for diagnosis. Early diagnosis of coronavirus plays an important role in preventing its spread and is essential for quarantine policies. For rapid decision and prompt triage of infected host, the immunochromatographic assay (ICA) has been widely used for point of care testing. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, it is necessary to use a mucolytic agent without affecting the antigens. In this study, we combined known mucolytic agents to lower the viscosity of sputum and applied that to alpha and beta coronavirus, porcine epidemic diarrhea virus (PEDV) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, spiked in sputum to find optimal pretreatment conditions. The pretreatment method using tris(2-carboxyethyl)phosphine (TCEP) and BSA was suitable for ICA diagnosis of sputum samples spiked with PEDV and MERS-CoV. This sensitive assay for the detection of coronavirus in sputum provides an useful information for the diagnosis of pathogen in low respiratory tract.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (29)
참고문헌 신청
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Omrani AS / 2015 / Middle East respiratory syndrome coronavirus(MERS-CoV) : animal to human interaction / Pathog Glob Health 109:354~362
World Health Organization / Middle East respiratory syndrome coronavirus (MERS-CoV)
van Boheemen S / 2012 / Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans / MBio 3:e00473~e00412
Annan A / 2013 / Human betacoronavirus 2c EMC/2012-related viruses in bats, Ghana and Europe / Emerg Infect Dis 19:456~459
Omrani AS / 2015 / Middle East respiratory syndrome coronavirus(MERS-CoV) : animal to human interaction / Pathog Glob Health 109:354~362
World Health Organization / Middle East respiratory syndrome coronavirus (MERS-CoV)
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