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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Mi-Sun (National Institute of Subtropical Agriculture, Rural Development Administration) Kim, Seong-Cheol (National Institute of Subtropical Agriculture, Rural Development Administration) Moon, Doo-Young (National Institute of Subtropical Agriculture, Rural Development Administration) Song, Kwan-Jeong (Faculty of Bioscience and Industry, Cheju National University)
저널정보
한국식물학회 식물학회지 식물학회지 제50권 제6호
발행연도
2007.1
수록면
681 - 686 (6page)

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Kiwifruit shoots can be rapidly propagated through a micro-cross section (MCS) system we established here. Optimal culture conditions were determined for different explant types, section sizes, and concentrations of inorganic salts and plant growth regulators. Rates of survival and callus formation were higher in half-strength MS salts than in full-strength MS media. Similar performance (i.e., survival and callus formation) was achieved with section sizes of either $800{\mu}m$ or $1200{\mu}m$. Proliferation efficiency was greatest when explants from stem tissue were cultured on 1/2 MS supplemented with $4.5{\times}10^{-3}{\mu}M$ 2,4-dichlo-rophenoxyacetic acid and $4.6{\times}10^{-1}{\mu}M$ zeatin. The number of shoots averaged 2.61 per explant, representing an efficiency of 94%. RAPD analysis revealed that the regenerated plants from our MCS system were genetically stable. These results show that the culturing of micro-cross sections from stem tissue is a powerful method for kiwifruit propagation.

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