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논문 기본 정보

자료유형
학술저널
저자정보
Wu, Chun-Hua (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) Murthy, Hosakatte Niranjana (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University) Hahn, Eun-Joo (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제30권 제8호
발행연도
2007.1
수록면
945 - 949 (5page)

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The aim of this study was to produce caffeic acid derivatives from adventitious root cultures of Echinacea purpurea, which are of high pharmaceutical value. The effects of both media optimization and replenishment strategies were adopted to achieve improved production of E. purpurea adventitious roots and caffeic acid derivatives. Of the different media strengths (0.25 MS, 0.5 MS, 0.75 MS and 1 MS) tested for the culturing of adventitious roots in 5 L bioreactors, 0.5 MS medium was found to be most suitable for the growth of adventitious roots. The optima accumulation of biomass (73.6 g $L^{-1}$ FW and 10.03 g $L^{-1}$ DW), phenolics (61.14 mg $g^{-1}$ DW) and flavonoids (38.30 mg $g^{-1}$ DW) were achieved in this medium. Furthermore, fed batch cultivations (media replenishment with 0.25 MS, 0.5 MS, 0.75 MS and 1 MS at the end of $2^{nd}$ and $3^{rd}$ weeks) to further enhance the production of adventitious root biomass and metabolites were also attempted. High adventitious root biomasses (83.1 g $L^{-1}$ FW and 15.30 g $L^{-1}$ DW) were achieved with feeding of the 0.5 MS medium at the end of $2^{nd}$ week. This led to slight decreases in the total production of phenolics and flavonoids; however, this feeding was responsible for increases in the accumulation of caftaric acid (5.76 mg $g^{-1}$ DW) and cichoric acid (26.12 mg $g^{-1}$ DW).

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