지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.10
- 수록면
- 278 - 288 (11page)
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초록· 키워드
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Background: Panax ginseng Meyer is a traditional medicinal plant famous for its strong therapeutic effects and serves as an important herbal medicine. To understand and manipulate genes involved in secondary metabolic pathways including ginsenosides, transcriptome profiling of P. ginseng is essential.
Methods: RNA-seq analysis of adventitious roots of two P. ginseng cultivars, Chunpoong (CP) and Cheongsun (CS), was performed using the Illumina HiSeq platform. After transcripts were assembled, expression profiling was performed.
Results: Assemblies were generated from w85 million and w77 million high-quality reads from CP and CS cultivars, respectively. A total of 35,527 and 27,716 transcripts were obtained from the CP and CS assemblies, respectively. Annotation of the transcriptomes showed that approximately 90% of the transcripts had significant matches in public databases.We identified several candidate genes involved in ginsenoside biosynthesis. In addition, a large number of transcripts (17%) with different gene ontology designations were uniquely detected in adventitious roots compared to normal ginseng roots.
Conclusion: This study will provide a comprehensive insight into the transcriptome of ginseng adventitious roots, and a way for successful transcriptome analysis and profiling of resource plants with less genomic information. The transcriptome profiling data generated in this study are available in our newly created adventitious root transcriptome database (http://im-crop.snu.ac.kr/transdb/index.php) for public use.
Methods: RNA-seq analysis of adventitious roots of two P. ginseng cultivars, Chunpoong (CP) and Cheongsun (CS), was performed using the Illumina HiSeq platform. After transcripts were assembled, expression profiling was performed.
Results: Assemblies were generated from w85 million and w77 million high-quality reads from CP and CS cultivars, respectively. A total of 35,527 and 27,716 transcripts were obtained from the CP and CS assemblies, respectively. Annotation of the transcriptomes showed that approximately 90% of the transcripts had significant matches in public databases.We identified several candidate genes involved in ginsenoside biosynthesis. In addition, a large number of transcripts (17%) with different gene ontology designations were uniquely detected in adventitious roots compared to normal ginseng roots.
Conclusion: This study will provide a comprehensive insight into the transcriptome of ginseng adventitious roots, and a way for successful transcriptome analysis and profiling of resource plants with less genomic information. The transcriptome profiling data generated in this study are available in our newly created adventitious root transcriptome database (http://im-crop.snu.ac.kr/transdb/index.php) for public use.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References
참고문헌 (50)
참고문헌 신청
1. [학술지(정기간행물)] - Wen J / 1996 / Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA / Mol Phylogenet Evol 6 : 167 ~ 177
2. [학술지(정기간행물)] - Hu SY / 1976 / The genus Panax (ginseng) in Chinese medicine / Econ Bot 30 : 11 ~ 28
3. [단행본] - Lee FC / 1992 / Facts about ginseng: the elixir of life / Hollym International Corporation Press
4. [학술지(정기간행물)] - Liu J / 1995 / Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly / Mech Ageing Dev 83 : 43 ~ 53
5. [학술지(정기간행물)] - Shin HR / 2000 / The cancer preventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
2. [학술지(정기간행물)] - Hu SY / 1976 / The genus Panax (ginseng) in Chinese medicine / Econ Bot 30 : 11 ~ 28
3. [단행본] - Lee FC / 1992 / Facts about ginseng: the elixir of life / Hollym International Corporation Press
4. [학술지(정기간행물)] - Liu J / 1995 / Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly / Mech Ageing Dev 83 : 43 ~ 53
5. [학술지(정기간행물)] - Shin HR / 2000 / The cancer preventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
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UCI(KEPA) : I410-ECN-0101-2018-524-001597479