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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,008 - 1,018 (11page)
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초록· 키워드
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Although bacteriocins with anti-listerial activity have been isolated from a wide variety of lactic acid bacteria, little is known about those from Leuconostoc lactis, a heterofermentative bacterium that produces diacetyl and exopolysaccharides in dairy foods. In this study, an anti-listerial bacteriocin was isolated from Leuc. lactis SD501 and characterized. It was particularly potent against Listeria monocytogenes and also inhibited Enterococcus faecalis. Anti-listerial activity reached a maximum during the early stationary phase and then decreased gradually. The anti-listerial substance was sensitive to proteinase K and ${\alpha}$-chymotrypsin, confirming its proteinaceous nature. Its activity remained stable at pH values ranging from 1 to 10. In addition, it was strongly resistant to high temperatures, retaining its activity even after incubation for 15 min at $121^{\circ}C$. The apparent molecular mass of the partially purified anti-listerial bacteriocin was approximately 7 kDa. The characteristics of the SD501 bacteriocin, including its small molecular size (<10 kDa), strong anti-listerial activity, wide pH stability and good thermostability, indicate its classification as a Class IIa bacteriocin.
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참고문헌 (50)
참고문헌 신청
Abriouel H / 2001 / Influence of physico-chemical factors on the oligomerization and biological activity of bacteriocin AS-48 / Curr Microbiol 42:89~95
Alvarez-Cisneros YM / 2010 / Biochemical characterization of a bacteriocin-like inhibitory substance produced by Enterococcus faecium MXVK29, isolated from Mexican traditional sausage / J Sci Food Agric 90:2475~2481
Alvarez-Sieiro P / 2016 / Bacteriocins of lactic acid bacteria : Extending the family / Appl Microbiol Biotechnol 100:2939~2951
Arakawa K / 2016 / Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag / Anim Sci J 87:449~456
Balay DR / 2017 / Purification of leucocin A for use on wieners to inhibit Listeria monocytogenes in the presence of spoilage organisms / Int J Food Microbiol 255:25~31
Alvarez-Cisneros YM / 2010 / Biochemical characterization of a bacteriocin-like inhibitory substance produced by Enterococcus faecium MXVK29, isolated from Mexican traditional sausage / J Sci Food Agric 90:2475~2481
Alvarez-Sieiro P / 2016 / Bacteriocins of lactic acid bacteria : Extending the family / Appl Microbiol Biotechnol 100:2939~2951
Arakawa K / 2016 / Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag / Anim Sci J 87:449~456
Balay DR / 2017 / Purification of leucocin A for use on wieners to inhibit Listeria monocytogenes in the presence of spoilage organisms / Int J Food Microbiol 255:25~31
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