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논문 기본 정보

자료유형
학술저널
저자정보
Park, Sung Hee (Department of Bioindustrial Technologies, Konkuk University) Kim, Jae-Hyeong (Department of Bioindustrial Technologies, Konkuk University) Min, Sang-Gi (Department of Bioindustrial Technologies, Konkuk University) Jo, Yeon-Ji (Department of Bioindustrial Technologies, Konkuk University) Chun, Ji-Yeon (Department of Bioindustrial Technologies, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제35권 제2호
발행연도
2015.1
수록면
265 - 271 (7page)

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In a previous study, hydrolysates of porcine placenta were obtained and the extraction efficiency for proteins and amino acids was compared between sub- and super-critical water extraction systems; optimum efficiency was found to be achieved using subcritical water ($170^{\circ}C$, 10 bar). In this study, the effects of adding ethanol to the subcritical water system were investigated. The lowest-molecular-weight extraction product detected weighed 434 Da, and the efficiency of extraction for low-molecular-weight products was increased when either the concentration of ethanol was decreased, or the extraction time was lengthened from 10 min to 30 min. The highest concentration of free amino acids (approximately 8 mM) was observed following 30 min extraction using pure distilled water. The concentration of free amino acids was significantly lower when ethanol was added or a shorter extraction time was used (p<0.05). Color change of the solution following extraction was measured. There were no significant differences in color between lysates produced with different extraction times when using distilled water (p>0.05); however, using different extraction times produced significant differences in color when using 20% or 50% ethanol solution for subcritical extraction (p<0.05). The range of pH for the hydrolysate solutions was 6.4-7.5. In conclusion, the investigated extraction system was successful in the extraction of $\leq$ 500 Da hydrolysates from porcine placenta, but addition of ethanol did not yield higher production of low-molecular-weight hydrolysates than that achieved by DW alone.

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