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This study was designed to optimize ethanol extraction process of unfertilized corn silk (UCS) to maximize phytochemical contents and bioactivities. The response surface methodology (RSM) with central composite design (CCD) was employed to obtain the optimal extraction conditions. The influence of ethanol concentration, extraction temperature and extraction time on total polyphenol contents, total flavonoid contents, maysin contents, 2,2-diphenyl-1- picrylhydrazyl(DPPH) radical scavenging activities and tyrosinase inhibition were analyzed. For all dependable variables, the most significant factor was ethanol concentration followed by extraction temperature and extraction time. The following optimum conditions were determined by simultaneous optimization of several responses with the Derringer’s desirability function using the numerical optimization function of the Design-Expert program: ethanol concentration 80.45%, extraction temperature 53.49oC, and extraction time 4.95 h. Under these conditions, the predicted values of total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity and tyrosinase inhibition were 2758.74 μg GAE/g dried sample, 1520.81 μg QUE/g dried sample, 810.26 mg/100g dried sample, 56.86% and 43.49%, respectively, and the overall desirability (D) was 0.74.
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참고문헌 (25)
참고문헌 신청
Chaiittianan R / 2016 / Anti-obesity potential of corn silk:Relationships of phytochemicals and antioxidation, anti-preadipocyte proliferation, anti-adipogenesis, and lipolysis induction / J. Funct. Food 23:497~510
Chen S / 2014 / Enzymolysis-ultrasonic assisted extraction: chemical characteristics and bioactivities of polysaccharides from corn silk / Carbohyd. Polym 101:332~341
Chew YL / 2009 / Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia / Food Chem 116:13~18
Choi DJ / 2014 / Neuroprotective effects of corn silk maysin via inhibition of H2O2- induced apoptotic cell death in SK-N-MC cells / Life Sci 109:57~64
Derrien M / 2017 / Optimization of a green process for the extraction of lutein and chlorophyll from spinach by-products using response surface methodology (RSM) / LWT-Food Sci. Technol 79:170~177
Chen S / 2014 / Enzymolysis-ultrasonic assisted extraction: chemical characteristics and bioactivities of polysaccharides from corn silk / Carbohyd. Polym 101:332~341
Chew YL / 2009 / Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia / Food Chem 116:13~18
Choi DJ / 2014 / Neuroprotective effects of corn silk maysin via inhibition of H2O2- induced apoptotic cell death in SK-N-MC cells / Life Sci 109:57~64
Derrien M / 2017 / Optimization of a green process for the extraction of lutein and chlorophyll from spinach by-products using response surface methodology (RSM) / LWT-Food Sci. Technol 79:170~177
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