Angelica Sinensis Polysaccharide Induces Erythroid Differentiation of Human Chronic Myelogenous Leukemia K562 Cells

Wang, Lu; Jiang, Rong; Song, Shu-Dan; Hua, Zi-Sen; Wang, Jian-Wei; Wang, Ya-Ping

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자료유형: 학술저널

저자정보: Wang, Lu (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Jiang, Rong (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Song, Shu-Dan (The First Affiliated Hospital of Chongqing Medical University) Hua, Zi-Sen (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Wang, Jian-Wei (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Wang, Ya-Ping (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제9호

발행연도: 2015.1

수록면: 3,715 - 3,721 (7page)

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Leukemia is a clonal disorder with blocked normal differentiation and cell death of hematopoietic progenitor cells. Traditional modalities with most used radiation and chemotherapy are nonspecific and toxic which cause adverse effects on normal cells. Differentiation inducing therapy forcing malignant cells to undergo terminal differentiation has been proven to be a promising strategy. However, there is still scarce of potent differentiation inducing agents. We show here that Angelica sinensis polysaccharide (ASP), a major active component in Dong quai (Chinese Angelica sinensis), has potential differentiation inducing activity in human chronic erythro-megakaryoblastic leukemia K562 cells. MTT assays and flow cytometric analysis demonstrated that ASP inhibited K562 cell proliferation and arrested the cell cycle at the G0/G1 phase. ASP also triggered K562 cells to undergo erythroid differentiaton as revealed by morphological changes, intensive benzidine staining and hemoglobin colorimetric reaction, as well as increased expression of glycophorin A (GPA) protein. ASP induced redistribution of STAT5 protein from the cytoplasm to the nucleus. Western blotting analysis further identified that ASP markedly sensitized K562 cells to exogenous erythropoietin (EPO) by activating EPO-induced JAK2/STAT5 tyrosine phosphorylation, thus augmenting the EPO-mediated JAK2/STAT5 signaling pathway. On the basis of these findings, we propose that ASP might be developed as a potential candidate for chronic myelogenous leukemia inducing differentiation treatment.

#Angelica sinensis polysaccharides #K562 #inducing differentiation #JAK2 #STAT5

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Wang, Lu

소속기관 Department of Oncology, Taizhou Second People’s Hospital, the Affiliated Hospital of Yangzhou Univer