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Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an autonomous vector DNA inside the host cell. This review introduces limitations and obstacles related to transformation technologies along with possible solutions. Current methods for cell wall removal and cell membrane permeabilization are summarized together with details of two popular technologies, Agrobacterium tumefaciens-mediated transformation and restriction enzyme-mediated integration.
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참고문헌 (86)
참고문헌 신청
Nevalainen H / 2014 / Making recombinant proteins in filamentous fungi: are we expecting too much? / Front Microbiol 5:75
Su X / 2012 / Heterologous gene expression in filamentous fungi / Adv Appl Microbiol 81:1~61
Singh SS / 2014 / Lectins from edible mushrooms / Molecules 20:446~469
Wösten HA / 2015 / Applications of hydrophobins:current state and perspectives / Appl Microbiol Biotechnol 99:1587~1597
El Enshasy HA / 2013 / Mushroom immunomodulators:unique molecules with unlimited applications / Trend Biotechnol 31:668~677
Su X / 2012 / Heterologous gene expression in filamentous fungi / Adv Appl Microbiol 81:1~61
Singh SS / 2014 / Lectins from edible mushrooms / Molecules 20:446~469
Wösten HA / 2015 / Applications of hydrophobins:current state and perspectives / Appl Microbiol Biotechnol 99:1587~1597
El Enshasy HA / 2013 / Mushroom immunomodulators:unique molecules with unlimited applications / Trend Biotechnol 31:668~677
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