Anti-inflammatory Effects of the Methanol Extract of Polytrichum Commune via NF-κB Inactivation in RAW 264.7 Macrophage Cells

Cho, Woong; Park, Seung-Jae; Shin, Ji-Sun; Noh, Young-Su; Cho, Eu-Jin; Nam, Jung-Hwan; Lee, Kyung-Tae

추천

검색

자료유형: 학술저널

저자정보: Cho, Woong (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) Park, Seung-Jae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) Shin, Ji-Sun (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) Noh, Young-Su (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) Cho, Eu-Jin (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) Nam, Jung-Hwan (Highland Agricultural Institute, Rural Development Adminstration) Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제16권 제4호

발행연도: 2008.1

수록면: 385 - 393 (9page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

As an attempt to search for bioactive natural products exerting anti-inflammatory activity, we evaluated the effects of the methanol extract of Polytrichum commune Hedw (PCM) (Polytrichaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines release in murine macrophage cell line RAW 264.7. PCM potently inhibits the production of NO, $PGE_2$, tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6. Consistent with these results, PCM also concentration-dependently inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygase (COX)-2 at the protein levels, and iNOS, COX-2, TNF-$\alpha$ and IL-6 at the mRNA levels without an appreciable cytotoxic effect on RAW 264.7 macrophag cells. Furthermore, PCM inhibited LPS-induced nuclear factor-kappa B (NF-$\kappa$B) activation as determined by NF-$\kappa$B reporter gene assay, and this inhibition was associated with a decrease in the nuclear translocation of p65 and p50 NF-$\kappa$B. Taken together, these results suggest that PCM may play an anti-inflammatory role in LPS-stimulated RAW 264.7 macrophages through the inhibitory regulation of iNOS, COX-2, TNF-$\alpha$ and IL-6 via NF-$\kappa$B inactivation.

#Polytrichum commune #Inducible nitric oxide synthase #iNOS) #Cyclooxygenase-2 #COX-2) #Nuclear factor- kappa B #NF- $kappa$B) #Anti-inflammation

참고문헌 (33)

참고문헌 신청

Aggarwal, B. B / 1996 / Tumor necrosis factors: developments during the last decade / Eur. Cytokine. Netw 7:93~124

Burg, N. D / 2001 / The neutrophil: function and regulation in innate and humoral immunity / Clin. Immunol 99:W7~W17

Chen, C. Y / 2007 / Luteolin suppresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages / Life Sci 81:1602~1614

Dinarello, C. A / 2004 / Infection, fever, and exogenous and endogenous pyrogens: some concepts have changed / J. Endotoxin. Res 10:201~222

Doyle, S. L / 2006 / Toll-like receptors: from the discovery of NFkappaB to new insights into transcriptional regulations in innate immunity / Biochem. Pharmacol 72:1102~1113

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!