지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Arabidopsis CYP51A2 (AtCYP51A2) mediates the sterol $14{\alpha}$-demethylation step in de novo sterol biosynthesis, and is constitutively and highly expressed in all plant tissues (Kim et al., 2005). We exploited the molecular features of its expression and the fundamental role of sterol biosynthesis in cells to develop a plant-derived promoter. Our GUS expression analysis between transgenic Arabidopsis lines for AtCYP51A2::GUS and 35S::GUS revealed that activity of the AtCYP51A2 promoter was comparable to that of the 35S promoter, based on enzymatic activities and protein levels. The AtCYP51A2 promoter was also constitutively active in transgenic tobacco, indicating that 5' regulatory elements could be conserved among CYP51 promoters in dicot plants. A homologue of AtCYP51A2 was identified from rape seed, a crop species closely related to Arabidopsis. Its constitutive tissue expression pattern implies that the application of this AtCYP51A2 promoter is possible for that species. Based on these results, we present a new binary vector system with the plant-derived AtCYP51A2 promoter, which is able to constitutively and ectopically drive a transgene in various dicotyledonous plants.
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