지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
We studied temporal and spatial expression patterns of the potato proteinase inhibitor II(PI-II) promoter, using transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants that carried of fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) gene. PI-II promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the PI-II promoter. We used several environmental stimuli to examine the induction of the PI-II promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the PI-II gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the PI-II promoter. A field strength of 0.75 kV/cm and 400 $\mu$F capacitance were optimal electroporation conditions for our transient assay.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
NcoI Restriction Fragment Length Polymorphism of the Tumor Necrosis Factor (TNF$\alpha$) Promoter in Korean
한국동물학회 학술대회
1995 .01
-
A Novel Bi-directional Promoter Cloned from Melon and Its Activity in Cucumber and Tobacco
식물학회지
2008 .01
-
Screening of Promoters from Metagenomic DNA and Their Use for the Construction of Expression Vectors
Journal of Microbiology and Biotechnology
2008 .01
-
Promoter effects of adeno-associated viral vector for transgeneexpression in the cochlea in vivo
Experimental and Molecular Medicine
2007 .01
-
Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori
생명과학회지
2011 .10
-
The Tissue Specific Expression of Wounding Inducible RCaM Promoter in Transgenic Tobacco Plants
한국생명과학회 심포지움
2004 .10
-
Developmentally Regulated Tissue-Specfic Expression of Wounding Inducible RCaM-2 Promoter in Transgenic Tobacco Plants
한국생명과학회 심포지움
2005 .04
-
Structural Characteristics of Two Wheat Histone H2A Genes Encoding Distinct Types of Variants and Functional Differences in Their Promoter Activity
한국식물학회 심포지움
1996 .01
-
프로모터 예측을 위한 다중 결정 모델 지능 시스템
한국생물정보시스템생물학회 학술대회
2003 .01
-
Building the Frequency Profile of the Core Promoter Element Patterns in the Three ChromHMM Promoter States at 200bp Intervals: A Statistical Perspective
Genomics & informatics
2015 .01
-
Functional Analysis of Pepper Cys₂/His₂-Type Zinc-Finger Protein Promoter Region in Response to Bacterial Infection and Abiotic Stresses in Tobacco Using Agrobacterium-Mediated Transient Assay
The Plant Pathology Journal
2005 .03
-
Transcription of Human Interleukin-2 Gene in Nicotiana tabacum Driven by CaMV35S Promoter
식물학회지
1997 .01
-
Studies on the Identification of Pythium spp. and Sclerotial Fungi isolated from Rice plants in Korea (II)
한국균학회지
1978 .01
-
Analysis of Hot Pepper Promoters and Gene Expression Regulation
한국유전체학회 Conference
2003 .01
-
Cell Cycle-Dependent Regulation of the Rat DNA Topoisomerase II $\alpha$ Gene Promoter
한국동물학회 학술대회
1998 .01
-
Characterization of an Apple Anthocyanidin Synthase Gene in Transgenic Tobacco Plants
식물학회지
2006 .01
-
Screening and Characteristic Analysis of Promoters from Plant Rhizobacteria
한국미생물학회 학술대회논문집
2009 .05
-
Expression of the Drosophila p38b Gene Promoter during Development and in the Immune Response
한국동물학회 학술대회
2003 .01
-
Transcriptional targeting of gene expression in breast cancerby the promoters of protein regulator of cytokinesis 1 andribonuclease reductase 2
Experimental and Molecular Medicine
2008 .01
-
Application of a Promoter Isolated from Chlorella Virus in Chlorella Transformation System
The Plant Pathology Journal
2004 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0