지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 345 - 353 (9page)
이용수
초록· 키워드
오류제보하기
For cancer gene therapy, cancer-specific overexpression of a therapeutic gene is required to reduce side effects derived from expression of the gene in normal cells. To develop such an expression vector, we searched for genes over-expressed and/or specifically expressed in cancer cells using bioinformatics and have selected genes coding for protein regulator of cytokinesis 1 (PRC1) and ribonuclease reductase 2 (RRM2) as candidates. Their cancer-specific expressions were confirmed in both breast cancer cell lines and patient tissues. We compared each promoter’s cancer-specific activity in the breast normal and cancer cell lines using the luciferase gene as a reporter and confirmed cancer-specific expression of both PRC1 and RRM2 promoters. To test activities of these promoters in viral vectors, the promoters were also cloned into an adeno-associated viral (AAV) vector containing green fluorescence protein (GFP) as the reporter. The GFP expression levels by these promoters were various depending on cell lines tested and, in MDA-MB-231 cells, GFP activities derived from the PRC1 and RRM2 promoters were as strong as that from the cytomegalovirus (CMV) promoter. Our result showed that a vector containing the PRC1 or RRM2 promoter could be used for breast cancer specific overexpression in gene therapy.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Ambrosini G / 1997 / A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma / Nat Med 3 : 917 ~ 21
Chen JS / 2004 / Cancer-specific activation of the survivin promoter and its potential use in gene therapy / Cancer Gene Ther 11 : 740 ~ 7
Chien PY / 1999 / A fusion protein of the estrogen receptor (ER) and nuclear receptor corepressor (NCoR) strongly inhibits estrogendependent responses in breast cancer cells
Dass CR / 2006 / Selective gene delivery for cancer therapy using cationic liposomes: in vivo proof of applicability / J Control Release 113 : 155 ~ 63
Dobson J / 2006 / Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery / Gene Ther 13 : 283 ~ 7
Chen JS / 2004 / Cancer-specific activation of the survivin promoter and its potential use in gene therapy / Cancer Gene Ther 11 : 740 ~ 7
Chien PY / 1999 / A fusion protein of the estrogen receptor (ER) and nuclear receptor corepressor (NCoR) strongly inhibits estrogendependent responses in breast cancer cells
Dass CR / 2006 / Selective gene delivery for cancer therapy using cationic liposomes: in vivo proof of applicability / J Control Release 113 : 155 ~ 63
Dobson J / 2006 / Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery / Gene Ther 13 : 283 ~ 7
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
폐암의 유전자 치료법을 위한 암특이적인 PRC1 프로모터
생명과학회지
2008 .10
-
Promoter effects of adeno-associated viral vector for transgeneexpression in the cochlea in vivo
Experimental and Molecular Medicine
2007 .01
-
Screening of Promoters from Metagenomic DNA and Their Use for the Construction of Expression Vectors
Journal of Microbiology and Biotechnology
2008 .01
-
Low-dose radiation response of the p21WAF1/CIP1 gene promoter transduced by adeno-associated virus vector
Experimental and Molecular Medicine
2006 .01
-
NcoI Restriction Fragment Length Polymorphism of the Tumor Necrosis Factor (TNF$\alpha$) Promoter in Korean
한국동물학회 학술대회
1995 .01
-
Analysis of Hot Pepper Promoters and Gene Expression Regulation
한국유전체학회 Conference
2003 .01
-
Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori
생명과학회지
2011 .10
-
Structural Characteristics of Two Wheat Histone H2A Genes Encoding Distinct Types of Variants and Functional Differences in Their Promoter Activity
한국식물학회 심포지움
1996 .01
-
Application of a Promoter Isolated from Chlorella Virus in Chlorella Transformation System
The Plant Pathology Journal
2004 .06
-
Expression of the Drosophila p38b Gene Promoter during Development and in the Immune Response
한국동물학회 학술대회
2003 .01
-
MCM promoter에서 E-box와 E2F 결합부위가 전사활성에 미치는 영향
생명과학회지
2004 .10
-
A Novel Bi-directional Promoter Cloned from Melon and Its Activity in Cucumber and Tobacco
식물학회지
2008 .01
-
Studies on the Foreign Gene Expression Using Fibroin Gene Promoter in Bombyx mori
한국동물학회 학술대회
2000 .01
-
Improved expression by cytomegalovirus promoter/enhancer and behavior of vascular endothelial growth factor gene after myocardial injection of naked DNA
Experimental and Molecular Medicine
2002 .01
-
Recombinant AAV Vector with MITF-M Promoter for Melanoma Gene Therapy
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2012 .03
-
Bioinformatics for the Cancer Gene Expression Profiling Studies
한국유전체학회 Conference
2003 .01
-
CaGe: A Web-Based Cancer Gene Annotation System for Cancer Genomics
Genomics & informatics
2012 .01
-
메타게놈에서 발굴한 프로모터를 장착한 새로운 항시발현 벡터의 가치평가
한국미생물·생명공학회지
2008 .01
-
Promoter Structure and Transcriptional Activity of Human Complement Receptor Type I (CR1) Gene
Korean journal of biological sciences
2003 .01
-
Bacillus thuringiensis cry1Aa1 Type Gene의 클로닝과 발현
한국미생물·생명공학회지
2004 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0