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자료유형
학술저널
저자정보
Kim, Mi-Jung (School of Life Sciences and Biotechnology, Korea University) Go, Young-Sam (Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute College of Agriculture and Life Sciences, Chonnam National University) Ahn, Sung-Ju (Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute College of Agriculture and Life Sciences, Chonnam National University) Chung, Chung-Han (Department of Biotechnology, Dong-A University) Suh, Mi-Chung (Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute College of Agriculture and Life Sciences, Chonnam National University)
저널정보
한국식물학회 식물학회지 식물학회지 제51권 제3호
발행연도
2008.1
수록면
174 - 179 (6page)

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Functional characterization of the fatty acid desaturase genes and seed-specific promoters is prerequisite for altering the unsaturated fatty acid content of oilseeds by genetic manipulation. The ${\omega}-6$ fatty acid desaturase (FAD2) and ${\omega}-3$ fatty acid desaturase (FAD3) catalyze extra-plastidial desaturation of oleic acid to linoleic acid and linoleic acid to linolenic acid, respectively. These are major constituents in seed storage oils. Here, we report the complementation of a perilla linoleic acid desaturase (PrFAD3) cDNA under the seed-specific sesame FAD2 (SeFAD2) promoter in the Arabidopsis fad3 mutant. PrFAD3 is functionally active and the SeFAD2 promoter is applicable for modifying fatty acid composition in developing seeds. Transient expression of the GUS gene under that promoter in the developing seeds and leaves of sesame, soybean, and corn via microprojectile bombardment indicated that the SeFAD2 promoter likely will be useful for altering the seed phenotypes of dicot and monocot crops.

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