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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제11호
- 발행연도
- 2010.1
- 수록면
- 1,555 - 1,562 (8page)
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초록· 키워드
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Development of the strain and the fermentation process of Hansenula polymorpha was implemented for the production of γ-linolenic acid (GLA, C18:3Δ6,9,12), an n-6polyunsaturated fatty acid (PUFA) that has been reported to possess a number of health benefits. The mutated Δ6-desaturase (S213A) gene of Mucor rouxii was expressed in H. polymorpha under the control of the methanol oxidase (MOX) promoter. Without the utilization of methanol, a high-cell-density culture of the yeast recombinant carrying the Δ6-desaturase gene was then achieved by fed-batch fermentation under glycerol-limited conditions. As a result, high levels of the Δ6-desaturated products,octadecadienoic acid (C18:2Δ6,9), GLA, and stearidonic acid (C18:4Δ6,9,12,15), were accumulated under the derepression conditions. The GLA production was also optimized by adjusting the specific growth rate. The results show that the specific growth rate affected both the lipid content and the fatty acid composition of the GLA-producing recombinant. Among the various specific growth rates tested, the highest GLA concentration of 697 mg/l was obtained in the culture with a specific growth rate of 0.08 /h. Interestingly, the fatty acid profile of the yeast recombinant bearing the Mucor Δ6-desaturase gene was similar to that of blackcurrant oil, with both containing similar proportions of n-3 and n-6 essential fatty acids.
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참고문헌 (23)
참고문헌 신청
Cahoon, E. B / 2007 / Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: Solving bottlenecks in fatty acid flux / Curr. Opin. Plant Bio
Castillo, M. L. R. D / 2004 / Fatty acid content and juice characteristics in black currant (Ribes nigrum L.) genotypes / J. Agric. Food Chem 52 : 948 ~ 952
Certik, M / 1999 / Biosynthesis and regulation of microbial polyunsaturated fatty acid production / J. Biosci. Bioeng 87 : 1 ~ 14
Das, U. N / 2004 / From bench to the clinic: γ-linolenic acid therapy of human gliomas. Prostaglandins Leukot / Essent. Fatty Acids 70 : 539 ~ 552
Dominguez, A / 1998 / Non-conventional yeasts as hosts for heterologous protein production / Int. Microbiol 1 : 131 ~ 142
Castillo, M. L. R. D / 2004 / Fatty acid content and juice characteristics in black currant (Ribes nigrum L.) genotypes / J. Agric. Food Chem 52 : 948 ~ 952
Certik, M / 1999 / Biosynthesis and regulation of microbial polyunsaturated fatty acid production / J. Biosci. Bioeng 87 : 1 ~ 14
Das, U. N / 2004 / From bench to the clinic: γ-linolenic acid therapy of human gliomas. Prostaglandins Leukot / Essent. Fatty Acids 70 : 539 ~ 552
Dominguez, A / 1998 / Non-conventional yeasts as hosts for heterologous protein production / Int. Microbiol 1 : 131 ~ 142
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