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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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To improve their growth and secondary metabolite production, we cultured Morinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm, as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or $45{\mu}moL\;m^{-2}s^{-1}$). Cell growth was greatest ($15.6g\;L^{-1}$ dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids) was maximized at 0.1 vvm. A PPF of $15{\mu}moL\;m^{-2}s^{-1}$ accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF ($45{\mu}moL\;m^{-2}s^{-1}$) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of light on cell growth and secondary metabolite contents in M. citrifolia cell cultures.
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