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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Ji Yoen (Dept. of Biology, College ofNatural Sciences, Busan National University) Lee, Mi Hwa (Dept. of Biology, College ofNatural Sciences, Busan National University) Park, Hae-Ryoun (Dept. ofDentistry, College ofDentistry, Busan National University) Choi, Jae Soo (Dept. ofChemistry, College ofNatural Sciences, Busan National University,) Seo, Hong Suk (Dept. ofBiology, College ofNatural Sciences, Kyungpook National University,) An, Won Gun Choi, Won Chul
저널정보
한국어병학회 한국어병학회지 한국어병학회지 제16권 제1호
발행연도
2003.1
수록면
39 - 49 (11page)

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Cytosolic oxidation by 4-hydroxy-2-nonenal (4HNE) and tert-butyl hydroperoxide (t-BHP) results in cell death of bovine aortic endothelial cells (BAEC). In this study, we have investigated the roles of antioxidants such as 2,3,6-tribromo-4,5-dihydroxy benzyl methyl ether (TDB) and phloroglucinol in preventing cell death. After treatment with oxidants for 6h, cells became compact and showed nuclear condensation, which were characteristics of early apoptosis. After l2h treatment, morphologic features including severe cytoplasm condensation, membrane blebbing, and apoptotic bodies were prominent and these findings were interpreted as characteristics of late-apoptosis. When the apoptotic cells were treated with antioxidants for 12h, both early and late apoptotic cells did show no significant change. After oxidant treated cells were incubated with antioxidant for 24h, the characteristics of early-apoptosis were eliminated but cells in lateapoptosis could not return to normal cells. These results suggest that TDB and phloroglucinol prevent the cells from dying through apoptosis induced by 4HNE and t-BHP in early stage.

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