지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.12
- 수록면
- 723 - 732 (10page)
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We investigated the inducing effects of Rubus coreanus extract (RCE) on apoptosis and its related gene expressions in human breast cancer cells. MDA-MB-231 cells were cultured in the presence of 0, 200, 300, and 400 ㎍/mL RCE for 24h. MTT assay demonstrated that relative cell viability measured a decrease in a dose-dependent manner (p＜0.05). This dependency was also found in the increasing levels of cell death by a dual staining with Hoechst 33322 and propidium iodide (p＜0.05). These close associations was also observed by different stages of apoptotic processes, as shown by an Apoptosis Detection Kit. To determine whether the alterations in such cell activities obtained above cause the induction of apoptotic genes, PT-PCR was performed expressions of both Bcl-2 and Bax mRNAs. The Bcl-2/Bax ratio which is an important indicator of apoptosis, was found to have significantly decreased dose dependence (p＜0.05). Western blot analysis also demonstrated that Caspase-3 significantly increases in a dose-dependent manner (p＜0.05) in addition to similar alterations of other proteins examined. Taken these results together, the ethanolic RCE used induces a reduction in cell viability along with increased membrane permeability. This leads to a precautious apoptotic process and, subsequently, cell death through the apoptotic pathway involving Bax and Caspase-3 in human breast cancer MDA-MB-231 cells.
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Abstract
서론
재료 및 방법
결과 및 고찰
결론 및 요약
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참고문헌 (42)
참고문헌 신청
1. [학술지(정기간행물)] - Aiyer HS / 2012 / Influence of berry polyphenols on receptor signaling and cell death pathways: Implications for breast cancer prevention / J Agric Food Chem 60 : 5693 ~ 5708
2. [단행본] - American Cancer Society / 2013 / Breast cancer: Facts & figures 2013-2014 / American Cancer Society Inc. : 4 ~ 11
4. [학술지(정기간행물)] - Belloc F / 1994 / A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells / Cytometry 17 : 59 ~ 65
5. [학술지(정기간행물)] - Bhandary B / 2012 / Water extracts of immature Rubus coreanus regulate lipid metabolism in liver cells / Biol Pharm Bull 35 : 1907 ~ 1913
6. [학술지(정기간행물)] - Boivin D / 2007 / Inhibition of cancer cell proliferation and suppre- ssion of TNFα-induced activation of NF-κB by edible berry juice / Anticancer Res 27 : 937 ~ 948
2. [단행본] - American Cancer Society / 2013 / Breast cancer: Facts & figures 2013-2014 / American Cancer Society Inc. : 4 ~ 11
4. [학술지(정기간행물)] - Belloc F / 1994 / A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells / Cytometry 17 : 59 ~ 65
5. [학술지(정기간행물)] - Bhandary B / 2012 / Water extracts of immature Rubus coreanus regulate lipid metabolism in liver cells / Biol Pharm Bull 35 : 1907 ~ 1913
6. [학술지(정기간행물)] - Boivin D / 2007 / Inhibition of cancer cell proliferation and suppre- ssion of TNFα-induced activation of NF-κB by edible berry juice / Anticancer Res 27 : 937 ~ 948
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UCI(KEPA) : I410-ECN-0101-2015-590-001038960