지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2000.1
- 수록면
- 273 - 278 (6page)
이용수
초록· 키워드
오류제보하기
Transforming growth factor-$\beta$ (IGF-$\beta$)s are multifunctional small polypeptides synthesized in most cell types. TGF-$\beta$ exerts pivotal effects on both bone formation and resorption. In addition, increasing lines of evidence implicate TGF-$\beta$ as a potential coupling factor between these two processes during bone remodeling. In the present study, the expression form and the activation mechanism of latent-TGF-$\beta$ were investigated using specific antibodies for each isoform. TGF-$\beta$s were observed to be synthesized and accumulated in a large amount in cultured osteoblastic cells. The estimated molecular weights of intracellular TGF-$\beta$2 and -$\beta$3 were 49 and 55 kDa, respectively. Based on proteolytic digestion study and immunofluorescence observation, these precursor forms seemed to be accumulated in distinct intracellular compartments. To examine whether the internal pool of TGF-$\beta$ was possiblely regulated by external signals, their biological activites were examined in a conditioned media of this cell. Although the intact conditioned media did not contain detectable TGF-$\beta$ activity, heat-treatment or acid-activation of the conditioned media revealed significant TGF-$\beta$ activity. Furthermore, in the presence of estrogen, this activity was dramatically diminished. It is known that activation of latent TGF-$\beta$ can be achieved by different chemical and enzymatic treatments, or by incubation with certain cell types. This extracellular activation was suggested as a key step in the regulation of TGF-$\beta$ activity. In addition to these extracellular activation, this study suggests that the synthesis and intracellular processing are important regulation steps for TGF-$\beta$ action. In addition, this regulation Is specific for TGF-$\beta$ type 2, because the change was not observed in TGF-$\beta$3 in osteoblastic cell line.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization of Apoptosis Induced by Transforming Growth Factor-$\beta$1(TGF-$\beta$1_ in Human Gastric Cancer Cells
한국동물학회 학술대회
1996 .01
-
INTRODUCTION OF NUTATED TGF-$\beta$1 cDNA CONFERS MACROPHAGE TO SECRETE AN ACTIVE FORM OF TGF- $\beta$1
한국동물학회 학술대회
1996 .01
-
Kinetics and Biological Function of Transforming Growth Factor-β Isoformsin Bovine and Human Colostrum
Journal of Microbiology and Biotechnology
2004 .01
-
TGF-β1 suppresses CCL3/4 expression through the ERK signaling pathway and inhibits intervertebral disc degeneration and inflammation-related pain in a rat model
Experimental and Molecular Medicine
2017 .01
-
Molecular Mechanisms of Microglial Deactivation by $TGF-{\beta}-inducible$ Protein ${\beta}ig-h3$
Integrative biosciences
2005 .01
-
Transforming Growth Factor-$\beta$2에 의한 연골세포 분화 촉진 효과
동물학회지
1995 .01
-
Regulation of IgA secretion by CD5 B cells : Possibile role of IL-5 and macrophage-derived TGF-$\beta$1
한국동물학회 학술대회
1998 .01
-
TGF-β1-induced PINCH-1-ILK-α-parvin complex formationregulates mesangial cell proliferation and hypertrophy
Experimental and Molecular Medicine
2007 .01
-
Transfection of CHl2.1LX B Cell Lymphoma with Porcine TGF-$\beta$1 cDNA
한국동물학회 학술대회
1995 .01
-
TGF-β3는 마우스 IgA, IgG2b 항체의 선택적 유도작용
미생물학회지
1999 .06
-
EpH4 세포에서 TGF-β에 의한 세포사멸시 Smad 단백질에 의존한 Gadd45b 유전자의 발현 변화
생명과학회지
2008 .04
-
Molecular Cloning and Characterization of Neuronal $\beta$-subunit of Large-Conductance$Ca^{2+}$-activated $K^+$ Channels from Rat Brain
한국생물물리학회 학술발표회
2001 .01
-
Functional Effects of $\beta4$-Subunit on Rat $BK_{Ca}$ Channel $\alpha$-Subunit, rSlo
한국생물물리학회 학술발표회
2002 .01
-
Expression and purification of immunologically active procine recombinant TGF-$\beta$1 and GFP fusion protein from insect cells
한국동물학회 학술대회
1999 .01
-
MACROPHAGE-DERIVED TGF-$\beta$1 ENHANCES IgA ISOTYPE SECRETION BY LPS-ACTIVATED PURINE B LYMPHOCYTES
한국동물학회 학술대회
1996 .01
-
TGF-β1 induces mouse dendritic cells to express VEGF and its receptor (Flt-1) under hypoxic conditions
Experimental and Molecular Medicine
2010 .01
-
Beta-voltaic battery and beta source using radioisotope Nickel
한국자기학회 학술연구발표회 논문개요집
2017 .11
-
Coordinated Spatial and Temporal Expression of Voltage-sensitive calcium Channel ${\alpha}_{1A}$ and $\beta_4$ Subunit mRNAs in Rat Cerebellum
Korean journal of biological sciences
1997 .01
-
Downregulation of SGK1 Expression is Critical for TGF-β-induced Apoptosis in Mouse Hepatocytes Cells
생명과학회지
2012 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0