지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1997.1
- 수록면
- 87 - 91 (5page)
이용수
초록· 키워드
오류제보하기
Effects of divalent cations such as $Mg^{2+}$, $Mn^{2+}$, $Ca^{2+}$, and $Zn^2$ on splicing activity of phage T4 thymidylate synthase intron RNA have been investigated. At the concentration of 0.5 mM, $Mn^{2+}$ in the absence of $Mg^{2+}$, a very small amount of pre-RNA was cleaved into ligation products (El-E2) but no circular or linear intron was produced. As the concentration of $Mn^{2+}$ was increased from 1 to 5 mM the pre-RNA was completely hydrolyzed. In the presence of 5 mM $Mg^{2+}$, both the linear intron and circular intron were produced but no El-E2 ligation product was produced. At both 3 and 5 mM $Mn^{2+}$ the RNA was hydrolyzed completely as observed with no $Mg^2+$ being present. In the case of $Zn^{2+}$, even at 0.5 mM concentration, the pre-RNA was completely hydrolyzed. This observation suggested that $Zn^{2+}$ facilitates RNA hydrolysis more rapidly than $Mn^{2+}$ does. at 5mM $Ca^{2+}$, the RNA was not hydrolyzed and remained intact as a primary transcript.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Supperessive Effects of Divalent Cations on Self-splicing Inhibition by Spectinomycin of Group 1 Intron RNA
The Journal of Microbiology
1999 .12
-
Role of Mg2+ in RNA Splicing of T4 td Intron
The Journal of Microbiology
1995 .06
-
In Vivo Target RNA Specificity of Trans-Splicing Phenomena by the Group I Intron
Genomics & informatics
2008 .01
-
Introns: The Functional Benefits of Introns in Genomes
Genomics & informatics
2015 .01
-
RNA-RNA Interactions between RNA Elements at the 5' end and at the Upstream of sgRNA of RNA Genome are Required for Potato virus X RNA Replication
The Plant Pathology Journal
2008 .09
-
Functional Modification of a Specific RNA with Targeted Trans-Splicing
Genomics & informatics
2004 .01
-
Pathogenic diversity of RNA variants and RNA variation-associated factors in cancer development
Experimental and Molecular Medicine
2020 .01
-
In vitro Synthesis of Ribonucleic Acids by T7 RNA Polymerase That was Fast Purified with a Modified Procedure
생명과학회지
2005 .10
-
Comparative Evaluation of Intron Prediction Methods and Detection of Plant Genome Annotation Using Intron Length Distributions
Genomics & informatics
2012 .01
-
K11 RNA 중합효소의 Cloning 및 발현
자연 과학 논문집 : 배재대학 첨단과학연구소
1997 .01
-
Effects of K+ Ion on in vitro RNA Splicing of T4 Phage Thymidylate Synthase Gene
The Journal of Microbiology
1996 .03
-
Tetrahymena thermophila의 group Ⅰ intron에 의한 trans-splicing 반응에 미치는 표적 RNA 구조의 영향분석
미생물학회지
1999 .09
-
Complete Genome Sequence of the RNAs 3 and 4 Segments of Rice stripe virus Isolates in Korea and their Phylogenetic Relationships with Japan and China Isolates
The Plant Pathology Journal
2009 .06
-
엔트로피 척도를 이용한 전체 RNA와 중폭 RNA의 유사성 분석
한국생물정보시스템생물학회 학술대회
2003 .01
-
Hybridization Detection of CMV Satellite RNA Using Enhanced Chemiluminescence
The Plant Pathology Journal
1990 .12
-
A Simple Procedure for RNA Isolation from Plants and Preservation of Plant Material for RNA Analysis
식물학회지
1987 .01
-
Biosynthesis of M1 RNA
생명과학
1992 .07
-
Phylogenetics and Gene Structure Dynamics of Polygalacturonase Genes in Aspergillus and Neurospora crassa
The Plant Pathology Journal
2013 .09
-
Validation of One-Step Real-Time RT-PCR Assay in Combination with Automated RNA Extraction for Rapid Detection and Quantiation of Hepatitis C Virus RNA for Routine Testing in Clinical Specimens
Journal of Microbiology and Biotechnology
2005 .01
-
Mutational Analysis of P2 Structure in T4 Phage td Intron
The Journal of Microbiology
1995 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0