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논문 기본 정보

자료유형
학술저널
저자정보
Yang, Long (Tobacco Laboratory, Shandong Agricultural University) Cho, Hwan-Gue (Graphics Application Laboratory, Department of Computer Science and Engineering, Pusan National University)
저널정보
한국유전체학회 Genomics & informatics Genomics & informatics 제10권 제1호
발행연도
2012.1
수록면
58 - 64 (7page)

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초록· 키워드

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Intron prediction is an important problem of the constantly updated genome annotation. Using two model plant (rice and $Arabidopsis$) genomes, we compared two well-known intron prediction tools: the Blast-Like Alignment Tool (BLAT) and Sim4cc. The results showed that each of the tools had its own advantages and disadvantages. BLAT predicted more than 99% introns of whole genomic introns with a small number of false-positive introns. Sim4cc was successful at finding the correct introns with a false-negative rate of 1.02% to 4.85%, and it needed a longer run time than BLAT. Further, we evaluated the intron information of 10 complete plant genomes. As non-coding sequences, intron lengths are not limited by a triplet codon frame; so, intron lengths have three phases: a multiple of three bases (3n), a multiple of three bases plus one (3n + 1), and a multiple of three bases plus two (3n + 2). It was widely accepted that the percentages of the 3n, 3n + 1, and 3n + 2 introns were quite similar in genomes. Our studies showed that 80% (8/10) of species were similar in terms of the number of three phases. The percentages of 3n introns in $Ostreococcus$ $lucimarinus$ was excessive (47.7%), while in $Ostreococcus$ $tauri$, it was deficient (29.1%). This discrepancy could have been the result of errors in intron prediction. It is suggested that a three-phase evaluation is a fast and effective method of detecting intron annotation problems.

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