지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Streptomyces actuosus DMCJ-49 and Streptomyces minoensis DMCJ-144 produce the .alpha.-amylase inhibitor. Inerspecific protoplast fusion technique was used to increase the productivity of .alpha.-amylase inhibitor. Four auxotrophic mutants were obtained respectively from two strains by N-methyl-N'-nitor-N-nitrosoguanidine(3mg/ml) treatment. The optimum conditions for the protoplast formation of Streptomyces actuosus DMCJ-49 ade was as follows; 1.2% w/v of glycine, 3mg/ml of lysozyme, and 30 min of lysozyme treatment followed by 36 hr. incubation in the protop-last formation medium. In case of DMCJ-144-his those were 1.2%w/v, 3 mg/ml, 30 minutes and 60 hours, respectively. Regeneration was accomplished with hypertonic soft agar medium that contained 0.4M sucrose, 20mM CaCl$_2$, 50 mM MgCl$_2$ and low levels of phosphate. Fusion of protoplasts carrying different auxotrophic markers was achieved by treatment with polyethylene glycol. The optimum concentration of polyethylene glycol 1450 for the production of recombinants was 40%w/v. When the protoplasts was treated with 40% polyethylene glycol for 30 minutes, the frequency of recombinants was 6.5$\times$$10^{-3}$ and the $\alpha$-amylase inhibition activity of $ade^-his^-$ No. 4, which is the fusant with the most improved activity increased from 33 to 125 I.U./ml.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
${\alpha}$-아밀라아제 저해제 생성 방선균, Streptomyces minoensis DMCJ-144의 저해제 생산을 위한 최적 배양 조건
약학회지
1992 .01
-
알파-아밀라제 저해제 생산 방선균 Streptomyces minoensis DMCJ-144의 균주개량
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
1993 .01
-
알파-아밀라제 저해제 생성 Streptomyces DMCJ-49의 동정과 저해제의 분리
약학회지
1989 .01
-
Studies on Screening and Isolation of .$\alpha$-Amylase Inhibitors of Soil Microorganisms (I)
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1985 .01
-
Genetic Transformation of Streptomyces caespitosus
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1993 .01
-
고온성 방사균에 의해 생산되는 α-amylase의 성질 ( Properties of α-amylase From Thermophilic Streptomyces sp. )
환경위생공학
1991 .01
-
Streptomyces sp.409 에서 분리한 항암활성 물질의 특징
약학회지
2000 .01
-
Purification and partial characterization of α-amylase from soybean (Glycine max)
Oriental pharmacy and experimental medicine
2004 .01
-
Purification and Biochemical Properties of Phospholipase D ($PLD_{57}$) Produced by Streptomyces sp. CS-57
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2007 .01
-
알파-아밀라제 저해제 생성 방선균의 검색
약학회지
1988 .01
-
이종간 원형질체 융합을 이용한 acetaminophen 생산균주 개량
약학회지
1994 .01
-
Precepitation and purification of amylase enzyme produced by streptomyces aureofaciens 77
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1990 .01
-
자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정
약학회지
1995 .01
-
Streptomyces Cytochrome P450 Enzymes and Their Roles in the Biosynthesis of Macrolide Therapeutic Agents
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Cathepsin B 저해물질을 생산하는 Streptomyces aburabiensis의 분리 및 동정
약학회지
1995 .01
-
The relationship between the level of salivary alpha amylase activity and pain severity in patients with symptomatic irreversible pulpitis
Restorative Dentistry and Endodontics
2013 .01
-
Activity and stability of purified amylase produced by streptomyces aureofaciens 77
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1990 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0