지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제30권 제10호
- 발행연도
- 2007.1
- 수록면
- 1,302 - 1,308 (7page)
이용수
초록· 키워드
오류제보하기
Streptomyces sp. CS-57, which was isolated from Korean soil, was found to produce phospholipase D ($PLD_{57}$) as an extracellular enzyme when cultured in medium containing 2% glucose, 1.5% yeast extract, 0.5% trypton, and 0.1% calcium carbonate at $28^{\circ}C$, and 160-rpm. $PLD_{57}$ was purified using Sepharose CL-6B column chromatography, and DEAE-Sepharose CL-6B ion exchange column chromatography. The specific activity of the purified enzyme increased 6.7 fold with 3% recovery. The purified enzyme was then analyzed using 12% SDS-PAGE, which revealed that the molecular mass of the purified enzyme was 55 kDa. $PLD_{57}$ showed both hydrolytic (H) and transphosphatidylation (T) activity, and the optimum temperatures of these activities were found to be $45^{\circ}C\;and\;35^{\circ}C$, respectively. Similarly, both of these activities were found to be optimal at a pH of 7.5. In addition, even after being heat treated at $45^{\circ}C$ for up to 2 h, the enzyme activity remained at 100%, and the H-activity was found to be stable at a pH of 6 to 8. Further, enzyme activity occurred in the presence of EDTA, indicating that metal ions are not required for their activity, although some metal ions did marginally increase the activity. Enzyme activity also increased by 75% in the presence of Triton-X 100 at a concentration of 0.375 %; however, none of the other detergents evaluated in this study were found to enhance enzyme activity.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Streptomyces sp.409 에서 분리한 항암활성 물질의 특징
약학회지
2000 .01
-
자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정
약학회지
1995 .01
-
Antifungal activity of chitinase from Streptomyces sp. Mong-20
대한약학회 학술대회
2001 .01
-
Streptomyces Cytochrome P450 Enzymes and Their Roles in the Biosynthesis of Macrolide Therapeutic Agents
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Antibacterial and Antitumor Macrolides from Streptomyces sp. Is9131
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2005 .01
-
Advanced Lipid Extraction Method for the Determination of the Phospholipase D Activity
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2003 .01
-
한국 Streptomyces SP.로부터 분리한 방향족 화합물과 지질 화합물의 세포독성 연구
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
1997 .01
-
Antitumor and Antioxidant Activity of Protocatechualdehyde Produced from Streptomyces lincolnensis M-20
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2008 .01
-
Regulation of extracellular N-acetyl-D-glucosamindiase production in the Streptomyces sp. 200803
대한약학회 학술대회
2001 .01
-
Precepitation and purification of amylase enzyme produced by streptomyces aureofaciens 77
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1990 .01
-
Cathepsin B 저해물질을 생산하는 Streptomyces aburabiensis의 분리 및 동정
약학회지
1995 .01
-
아데노신 탈아미노화 효소 억제제를 생산하는 Streptomyces sp. V-8의 변이종으로부터 페녹사지논 합성효소의 분리 및 특성
약학회지
1999 .01
-
C6세포에서 phospholipase $A_2$활성에 대한 ATP의 작용
약학회지
2001 .01
-
사람 양수중 다종의 세포외성 포스포리파제의 $A_2$의 부분정제 및 특성
약학회지
1997 .01
-
방선균 분리주 No. 2511-5로부터 포스포리파제 C 저해물질 스코파훈진의 분리
약학회지
1995 .01
-
Topoisomerase I Inhibitors from the Streptomyces sp. Strain KM86-9B Isolated from a Marine Sponge
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1998 .01
-
$\alpha$-Amylase 저해제 생성균 Streptomyces actuosus DMCJ-49와 Streptomyces minoensis DMCJ-144의 종간 융합에 의한 균주 개량
약학회지
1991 .01
-
Genetic Transformation of Streptomyces caespitosus
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1993 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0