지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: When administered soon after menopause, hormone therapy can prevent coronary heart diseases in women. To explore the mechanism underlying the cardioprotective actions of estrogen, we investigated the effects of 17β-estradiol (17β-E2) on the plasminogen activator system using cultured vascular smooth muscle cells (VSMCs). Methods: VSMCs were isolated from rat aortas. Protein expression of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were evaluated using Western blotting and enzyme-linked immunosorbent assay, respectively. The enzyme activity of PAI-1 in a conditioned medium was assessed via reverse fibrin overlay zymography and that of t-PA was assessed via fibrin overlay zymography. Gene expression was quantified using real-time reverse transcription-polymerase chain reaction. Results: Following pre-treatment for 24 hours, 17β-E2 suppressed both protein expression and enzyme activity of PAI-1 stimulated by lysophosphatidylcholine (lysoPC) in a significant and dose-dependent manner at a near physiological concentration. Moreover, 17β-E2 (10−7 M) inhibited PAI-1 gene expression, and ICI 182,780—a specific estrogen receptor antagonist—blocked the effects of 17β-E2 on the PAI-1 protein. 17β-E2 did not affect t-PA secretion but significantly enhanced free t-PA activity through reduced binding to PAI-1. Furthermore, 17β-E2 suppressed intracellular reactive oxygen species production and nuclear factor-κB-mediated transcription. Conclusions: In VSMCs stimulated with lysoPC, 17β-E2 reduced PAI-1 expression through a non-receptor-mediated mechanism via antioxidant activity as well as a receptor-mediated mechanism; however, it did not alter t-PA secretion. Of note, 17β-E2 suppressed PAI- 1 activity and concurrently enhanced t-PA activity, suggesting a beneficial influence on fibrinolysis.
목차
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참고문헌 (23)
참고문헌 신청
Ibrahim H / 2017 / Platelet pathophysiology, pharmacology, and function in coronary artery disease / Coron Artery Dis 28:614~623
Sobel BE / 2003 / Intramural plasminogen activator inhibitor type-1 and coronary atherosclerosis / Arterioscler Thromb Vasc Biol 23:1979~1989
Ilić M / 2002 / Plasminogen activator inhibitor in patients with acute myocardial infarction and re-infarction in syndrome X / Clin Lab 48:125~128
Rossouw JE / 2007 / Postmenopausal hormone therapy and risk of cardiovascular disease by age and years since menopause / JAMA 297:1465~1477
Boardman HM / 2015 / Hormone therapy for preventing cardiovascular disease in post-menopausal women / Cochrane Database Syst Rev (3):CD002229
Sobel BE / 2003 / Intramural plasminogen activator inhibitor type-1 and coronary atherosclerosis / Arterioscler Thromb Vasc Biol 23:1979~1989
Ilić M / 2002 / Plasminogen activator inhibitor in patients with acute myocardial infarction and re-infarction in syndrome X / Clin Lab 48:125~128
Rossouw JE / 2007 / Postmenopausal hormone therapy and risk of cardiovascular disease by age and years since menopause / JAMA 297:1465~1477
Boardman HM / 2015 / Hormone therapy for preventing cardiovascular disease in post-menopausal women / Cochrane Database Syst Rev (3):CD002229
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