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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 253 - 258 (6page)
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초록· 키워드
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Methods for reproducibly isolating and enriching small extracellular vesicles (EVs) from blood are essential for clinical utilization of small EVs in cancer patients. We combined ultracentrifugation (UC) with polymer-based precipitation (ExoQuick [EQ] or Total Exosome Isolation [TEI] kit) to isolate small EVs (diameter, 30–150 nm) from the serum of breast cancer patients. We compared the performance of four cycles of UC (UC4x) with that of two cycles of UC followed by enrichment using the EQ (UC2x→EQ) or TEI (UC2x→TEI) kits. The mean concentration of small EVs isolated from 1 mL of serum using UC2x→EQ (139.0±29.1 μg) and UC2x→TEI (140.4±5.0 μg) did not differ from that obtained using UC4x (141.8±26.9 μg). The mean number of EV particles obtained using UC4x was 29.2± 9.9×109 per mL of serum, whereas UC2x→EQ and UC2x→TEI yielded higher numbers of EVs (50.7±17.0×109 and 59.3±20.6×109, respectively). Concentrations of EV microRNAs, including miR-21 and miR-155, did not differ between the three methods. In conclusion, performing UC prior to the use of polymer-based precipitation kits could be feasible for isolating small EVs from human serum in large sample-based translational researches.
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등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Sharma A / 2016 / Tumor exosomes : cellular postmen of cancer diagnosis and personalized therapy / Nanomedicine (Lond) 11:421~437
EL Andaloussi S / 2013 / Extracellular vesicles : biology and emerging therapeutic opportunities / Nat Rev Drug Discov 12:347~357
Tkach M / 2016 / Communication by extracellular vesicles : where we are and where we need to go / Cell 164:1226~1232
Cocucci E / 2015 / Ectosomes and exosomes : shedding the confusion between extracellular vesicles / Trends Cell Biol 25:364~372
Raposo G / 2013 / Extracellular vesicles: exosomes, microvesicles, and friends / J Cell Biol 200:373~383
EL Andaloussi S / 2013 / Extracellular vesicles : biology and emerging therapeutic opportunities / Nat Rev Drug Discov 12:347~357
Tkach M / 2016 / Communication by extracellular vesicles : where we are and where we need to go / Cell 164:1226~1232
Cocucci E / 2015 / Ectosomes and exosomes : shedding the confusion between extracellular vesicles / Trends Cell Biol 25:364~372
Raposo G / 2013 / Extracellular vesicles: exosomes, microvesicles, and friends / J Cell Biol 200:373~383
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