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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제1호
- 발행연도
- 2020.1
- 수록면
- 127 - 135 (9page)
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Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.
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참고문헌 (46)
참고문헌 신청
Wagner EGH / 2015 / Small RNAs in bacteria and archaea : who they are, what they do, and how they do it / Adv. Genet 90:133~208
Melamed S / 2016 / Global mapping of small RNA-target interactions in bacteria / Mol. Cell 63:884~897
Frohlich KS / 2018 / Small regulatory RNAs in the enterobacterial response to envelope damage and oxidative stress / Microbiol. Spectr. 6
Kavita K / 2018 / New aspects of RNA-based regulation by Hfq and its partner sRNAs / Curr. Opin. Microbiol 42:53~61
Gimpel M / 2016 / Dual-function sRNA encoded peptide SR1P modulates moonlighting activity of B. subtilis GapA / RNA Biol. 13:916~926
Melamed S / 2016 / Global mapping of small RNA-target interactions in bacteria / Mol. Cell 63:884~897
Frohlich KS / 2018 / Small regulatory RNAs in the enterobacterial response to envelope damage and oxidative stress / Microbiol. Spectr. 6
Kavita K / 2018 / New aspects of RNA-based regulation by Hfq and its partner sRNAs / Curr. Opin. Microbiol 42:53~61
Gimpel M / 2016 / Dual-function sRNA encoded peptide SR1P modulates moonlighting activity of B. subtilis GapA / RNA Biol. 13:916~926
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