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논문 기본 정보
- 자료유형
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제11호
- 발행연도
- 2019.1
- 수록면
- 1,717 - 1,728 (12page)
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이 논문의 연구 히스토리 (2)
2019
Construction of Recombinant Bifidobacterium bifidum BGN4 Expressing Bifidobacterial β-Galactosidase Gene and Application for Improvement of Lactose Intolerance
박민주
식품영양학과
2019.01
학위논문
Cloning and Heterologous Expression of the β-Galactosidase Gene from Bifidobacterium longum RD47 in B. bifidum BGN4
박민주
,
박명수
,
지근억
Journal of Microbiology and Biotechnology
2019.01
학술저널
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초록· 키워드
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The gene encoding β-galactosidase was cloned from Bifidobacterium longum RD47 with combinations of several bifidobacterial promoters, and expressed in B. bifidum BGN4. Among the recombinant bifidobacteria, BGN4+G1 showed the highest β-galactosidase level, for which the hydrolytic activity was continuously 2.5 to 4.2 times higher than that of BGN4 and 4.3 to 9.6 times higher than that of RD47. The β-galactosidase activity of BGN4+G1 was exceedingly superior to that of any of the other 35 lactic acid bacteria. When commercial whole milk and BGN4+G1 were reacted, BGN4+G1 removed nearly 50% of the lactose in the milk by the 63-h time point, and a final 61% at 93 h. These figures are about twice the lactose removal rate of conventional fermented milk. As for the reaction of commercial whole milk and crude enzyme extract from BGN4+G1, the β-galactosidase of BGN4+G1 eliminated 51% of the lactose in milk in 2 h. As shown below, we also compared the strengths and characteristics of the strong bifidobacterial promoters reported by previous studies.
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참고문헌 (65)
참고문헌 신청
Nguyen T-T / 2015 / Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum : effect of different parameters on the sakacin Pbased expression system / Microb. Cell Fact 14:30
Yuan T / 2008 / Heterologous expression of a gene encoding a thermostable β-galactosidase from Alicyclobacillus acidocaldarius / Biotechnol. Lett 30:343~348
Kamran A / 2016 / Lactose hydrolysis approach : isolation and production of βgalactosidase from newly isolated Bacillus strain B-2 / Biocatal. Agric. Biotechnol 5:99~103
Nguyen TH / 2006 / Purification and characterization of two novel β-galactosidases from Lactobacillus reuteri / J. Agric. Food Chem 54:4989~4998
Splechtna B / 2007 / Process development for the production of prebiotic galacto-oligosaccharides from lactose using βgalactosidase from Lactobacillus sp / Biotechnol. J 2:480~485
Yuan T / 2008 / Heterologous expression of a gene encoding a thermostable β-galactosidase from Alicyclobacillus acidocaldarius / Biotechnol. Lett 30:343~348
Kamran A / 2016 / Lactose hydrolysis approach : isolation and production of βgalactosidase from newly isolated Bacillus strain B-2 / Biocatal. Agric. Biotechnol 5:99~103
Nguyen TH / 2006 / Purification and characterization of two novel β-galactosidases from Lactobacillus reuteri / J. Agric. Food Chem 54:4989~4998
Splechtna B / 2007 / Process development for the production of prebiotic galacto-oligosaccharides from lactose using βgalactosidase from Lactobacillus sp / Biotechnol. J 2:480~485
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