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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제5호
- 발행연도
- 2017.1
- 수록면
- 909 - 915 (7page)
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초록· 키워드
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Bifidobacterium bifidum BGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains of B. bifidum and 75 strains of the other Bifidobacterium species. The BGN4-specific regions were derived using megaBLAST against genome sequences of various B. bifidum databases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was 2.8 × 101 CFU/ml of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4- fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.
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참고문헌 (22)
참고문헌 신청
Arboleya S / 2010 / Characterization and in vitro properties of potentially probiotic Bifidobacterium strains isolated from breast-milk / Int. J. Food Microbiol 149 : 28 ~ 36
Coudeyras S / 2008 / Taxonomic and strain-specific identification of the probiotic strain Lactobacillus rhamnosus 35 within the Lactobacillus casei group / Appl. Environ. Mi
Endo A / 2012 / Evaluation of strainspecific primers for identification of Lactobacillus rhamnosus GG / FEMS Microbiol. Lett 337 : 120 ~ 125
Frick JS / 2007 / Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells / Inflamm. Bowel Dis 13 : 83 ~ 90
Fujimoto J / 2011 / Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide
Coudeyras S / 2008 / Taxonomic and strain-specific identification of the probiotic strain Lactobacillus rhamnosus 35 within the Lactobacillus casei group / Appl. Environ. Mi
Endo A / 2012 / Evaluation of strainspecific primers for identification of Lactobacillus rhamnosus GG / FEMS Microbiol. Lett 337 : 120 ~ 125
Frick JS / 2007 / Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells / Inflamm. Bowel Dis 13 : 83 ~ 90
Fujimoto J / 2011 / Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide
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