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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 24 - 31 (8page)
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Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK’s) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.
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참고문헌 (27)
참고문헌 신청
Zhao H / 2006 / Liquid methanol concentration sensors for direct methanol fuel cells / J. Power Sources 159:626~636
Okada T / 1997 / Ion and Water Transport Characteristics in Membranes for Polymer Electrolyte Fuel Cells Containing H+ and Ca2+ Cations / J. Electrochem. Soc 14:2744~2750
Laub MT / 2007 / Specificity in two-component signal transduction pathways / Annu. Rev. Genet 41:121~145
Stock AM / 2000 / Two-Component Signal Transduction / Annu. Rev. Biochem 69:183~215
Mitrophanov AY / 2008 / Signal integration in bacterial two-component regulatory systems / Genes Dev 22:2601~2611
Okada T / 1997 / Ion and Water Transport Characteristics in Membranes for Polymer Electrolyte Fuel Cells Containing H+ and Ca2+ Cations / J. Electrochem. Soc 14:2744~2750
Laub MT / 2007 / Specificity in two-component signal transduction pathways / Annu. Rev. Genet 41:121~145
Stock AM / 2000 / Two-Component Signal Transduction / Annu. Rev. Biochem 69:183~215
Mitrophanov AY / 2008 / Signal integration in bacterial two-component regulatory systems / Genes Dev 22:2601~2611
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