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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제6호
- 발행연도
- 2017.6
- 수록면
- 1,106 - 1,111 (6page)
- DOI
- 10.4014/jmb.1611.11070
이용수
초록· 키워드
오류제보하기
Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Cybulski A. / 1994 / Liquid-phase methanol synthesis: catalysts, mechanism, kinetics, chemical equilibria, vapor-liquid equilibria, and modeling — a review / Catal. Rev 36:557~615
Arakawa H. / 1998 / Research and development on new synthetic routes for basic chemicals by catalytic hydrogenation of CO2 / Stud. Surf. Sci. Catal 114:19~30
Marchionna M / 1998 / A review of low-temperature methanol synthesis / Stud. Surf. Sci. Catal 119:539~544
Rozovskii AY / 1999 / Catalytic synthesis of methanol / Kinet. Catal 40:773~794
Lange J-P / 2001 / Methanol synthesis: a short review of technology improvements / Catal. Today 64:3~8
Arakawa H. / 1998 / Research and development on new synthetic routes for basic chemicals by catalytic hydrogenation of CO2 / Stud. Surf. Sci. Catal 114:19~30
Marchionna M / 1998 / A review of low-temperature methanol synthesis / Stud. Surf. Sci. Catal 119:539~544
Rozovskii AY / 1999 / Catalytic synthesis of methanol / Kinet. Catal 40:773~794
Lange J-P / 2001 / Methanol synthesis: a short review of technology improvements / Catal. Today 64:3~8
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