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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 546 - 554 (9page)
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초록· 키워드
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This study, for the first time, reports the functional expression of lipase B derived from the yeast Candida antarctica (CALB) in Corynebacterium strain using the Escherichia coli plasmid PK18. The CALB gene fragment encoding a 317-amino-acid protein was successfully obtained from the total RNA of C. antarctica. CALB was readily produced in the Corynebacterium strain without the use of induction methods described in previous studies. This demonstrated the extracellular production of CALB in the Corynebacterium strain. CALB produced in the Corynebacterium MB001 strain transformed with pEC-CALB recombinant plasmid exhibited maximum extracellular enzymatic activity and high substrate affinity. The optimal pH and temperature for the hydrolysis of 4-nitrophenyl laurate by CALB were 9.0 and 40℃, respectively. The enzyme was stable at pH 10.7 in the glycine-KOH buffer and functioned as an alkaline lipase. The CALB activity was inhibited in the presence of high concentration of Mg2+, which indicated that CALB is not a metalloenzyme. These properties are key for the industrial application of the enzyme.
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참고문헌 (34)
참고문헌 신청
Anné J / 2014 / Protein secretion biotechnology in Gram-positive bacteria with special emphasis on Streptomyces lividans / Biochim. Biophys. Acta 1843:1750~1761
Uppenberg J / 1994 / The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica / Curr. Biol 2:293~308
Emond S / 2010 / New efficient recombinant expression system to engineer Candida antarctica lipase B / Appl. Environ. Microbiol 76:2684~2687
Liu ZQ / 2012 / Cloning, expression and characterization of a lipase gene from the Candida antarctica ZJB09193 and its application in biosynthesis of vitamin A esters / Microbiol. Res 167:452~460
Mertens N / 1995 / Tight transcriptional control mechanism ensures stable high-level expression from T7 promoterbased expression plasmids / Biotechnology 13:175~179
Uppenberg J / 1994 / The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica / Curr. Biol 2:293~308
Emond S / 2010 / New efficient recombinant expression system to engineer Candida antarctica lipase B / Appl. Environ. Microbiol 76:2684~2687
Liu ZQ / 2012 / Cloning, expression and characterization of a lipase gene from the Candida antarctica ZJB09193 and its application in biosynthesis of vitamin A esters / Microbiol. Res 167:452~460
Mertens N / 1995 / Tight transcriptional control mechanism ensures stable high-level expression from T7 promoterbased expression plasmids / Biotechnology 13:175~179
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