Studies of the effects and mechanisms of ginsenoside Re and Rk₃ on myelosuppression induced by cyclophosphamide

Jiahong Han; Jing Xia; Lianxue Zhang; Enbo Cai; Yan Zhao; Xuan Fei; Xiaohuan Jia; He Yang; Shuangli Liu

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자료유형: 학술저널

저자정보: Jiahong Han (Jilin Agricultural University) Jing Xia (Jilin Agricultural University) Lianxue Zhang (Jilin Agricultural University) Enbo Cai (Jilin Agricultural University) Yan Zhao (Jilin Agricultural University) Xuan Fei (Jilin Agricultural University) Xiaohuan Jia (Jilin Agricultural University) He Yang (Jilin Agricultural University) Shuangli Liu (Jilin Agricultural University)

저널정보: 고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.43 No.4

발행연도: 2019.10

수록면: 618 - 624 (7page)

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Background: Ginsenoside Re (Re) is one of the major components of Panax ginseng Meyer. Ginsenoside Rk₃ (Rk₃) is a secondary metabolite of Re. The aim of this study was to investigate and compare the effects and underlying mechanisms of Re and Rk₃ on cyclophosphamide-induced myelosuppression.
Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide. Peripheral blood cells, bone marrow nucleated cells, and colony yield of hematopoietic progenitor cells in vitro were counted. The levels of erythropoietin, thrombopoietin, and granulocyte macrophage colony-stimulating factor in plasma were measured by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. The expression of apoptotic protein bcl-2, bax, and caspase-3 was detected by Western blotting.
Results: Both Re and Rk₃ could improve peripheral blood cells, bone marrow nucleated cell counts, thymus index, and spleen index. Furthermore, they could enhance the yield of colonies cultured in vitro and make the levels of granulocyte macrophage colony-stimulating factor, erythropoietin, and thrombopoietin normal, reduce the ratio of G₀/G₁ phase cells, and increase the proliferation index. Finally, Re and Rk₃ could upregulate the expression of bcl-2, whereas they could downregulate the expression of bax and caspase-3.
Conclusion: Re and Rk₃ could improve the hematopoietic function of myelosuppressed mice. The effect of Rk₃ was superior to that of Re at any dose. Regulating the levels of cytokines, promoting cells enter the normal cell cycle, regulating the balance of bcl-2/bax, and inhibiting the expression of caspase-3 may be the effects of Re and Rk₃ on myelosuppression.

#Chemotherapy #Ginsenoside Re #Ginsenoside Rk₃ #Myelosuppression

참고문헌 (43)

참고문헌 신청

Hoekman K / 1991 / Effects of recombinant human granulocyte-macrophage colonystimulating factor on myelosuppression induced by multiple cycles of highdose chemotherapy in patients with advanced breast cancer / J Natl Cancer Inst 83(21):1546~1553

Molyneux G / 2011 / Haemotoxicity of busulphan, doxorubicin, cisplatin and cyclophosphamide in the female BALB/c mouse using a brief regimen of drug administration / Cell Biol Toxicol 27(1):13~40

Toussaint O / 2000 / Cellular and molecular mechanisms of stress-induced premature senescence(SIPS)of human diploid fibroblasts and melanocytes / Exp Gerontol 35(8):927~945

Wickremasinghe RG / 1999 / Biochemical and genetic control of apoptosis : relevance to normal hematopoiesis and hematological malignancies / Blood 93(11):3587~3600

Friberg LE / 2003 / Mechanistic models for myelosuppression / Invest New Drugs 21(2):183

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