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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 233 - 239 (7page)
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초록· 키워드
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Background: Nephrotoxicity is the major side effect in cisplatin chemotherapy. Previously, we reported that the ginsenosides Rk3 and Rh4 reduced cisplatin toxicity on porcine renal proximal epithelial tubular cells(LLC-PK1). Here, we aimed to evaluate the protective effect of ginsenosides Rk3 and Rh4 on kidney function and elucidate their antioxidant effect using in vitro and in vivomodels of cisplatin-induced acute renal failure.
Methods: An enriched mixture of ginsenosides Rk3 and Rh4 (KG-KH; 49.3% and 43.1%, respectively) was purified from sun ginseng (heat processed Panax ginseng). Cytotoxicity was induced by treatment of 20mM cisplatin to LLC-PK1 cells and rat model of acute renal failure was generated by single intraperitoneal injection of 5 mg/kg cisplatin. Protective effects were assessed by determining cell viability, reactive oxygen species generation, blood urea nitrogen, serum creatinine, antioxidant enzyme activity, and histopathological examination.
Results: The in vitro assay demonstrated that KG-KH (50 mg/mL) significantly increased cell viability (4.6-fold), superoxide dismutase activity (2.8-fold), and glutathione reductase activity (1.5-fold), but reduced reactive oxygen species generation (56%) compared to cisplatin control cells. KG-KH (6 mg/kg, per os) also significantly inhibited renal edema (87% kidney index) and dysfunction (71.4% blood urea nitrogen, 67.4% creatinine) compared to cisplatin control rats. Of note, KG-KH significantly recovered the kidney levels of catalase (1.2-fold) and superoxide dismutase (1.5-fold).
Conclusion: Considering the oxidative injury as an early trigger of cisplatin nephrotoxicity, our findings suggest that ginsenosides Rk3 and Rh4 protect the kidney from cisplatin-induced oxidative injury and help to recover renal function by restoring intrinsic antioxidant defenses.
Methods: An enriched mixture of ginsenosides Rk3 and Rh4 (KG-KH; 49.3% and 43.1%, respectively) was purified from sun ginseng (heat processed Panax ginseng). Cytotoxicity was induced by treatment of 20mM cisplatin to LLC-PK1 cells and rat model of acute renal failure was generated by single intraperitoneal injection of 5 mg/kg cisplatin. Protective effects were assessed by determining cell viability, reactive oxygen species generation, blood urea nitrogen, serum creatinine, antioxidant enzyme activity, and histopathological examination.
Results: The in vitro assay demonstrated that KG-KH (50 mg/mL) significantly increased cell viability (4.6-fold), superoxide dismutase activity (2.8-fold), and glutathione reductase activity (1.5-fold), but reduced reactive oxygen species generation (56%) compared to cisplatin control cells. KG-KH (6 mg/kg, per os) also significantly inhibited renal edema (87% kidney index) and dysfunction (71.4% blood urea nitrogen, 67.4% creatinine) compared to cisplatin control rats. Of note, KG-KH significantly recovered the kidney levels of catalase (1.2-fold) and superoxide dismutase (1.5-fold).
Conclusion: Considering the oxidative injury as an early trigger of cisplatin nephrotoxicity, our findings suggest that ginsenosides Rk3 and Rh4 protect the kidney from cisplatin-induced oxidative injury and help to recover renal function by restoring intrinsic antioxidant defenses.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (40)
참고문헌 신청
[학술지(정기간행물)] - Lebwohl D / 1998 / Clinical development of platinum complexes in cancer therapy : an historical perspective and an update / Eur J Cancer 34 : 1522 ~ 1534
[학술지(정기간행물)] - Arany I / 2003 / Cisplatin nephrotoxicity / Semin Nephrol 23 : 460 ~ 464
[학술지(정기간행물)] - Pabla N / 2008 / Cisplatin nephrotoxicity : mechanisms and renoprotective strategies / Kidney Int 73 : 994 ~ 1007
[학술지(정기간행물)] - Wang D / 2005 / Cellular processing of platinum anticancer drugs / Nat Rev Drug Discov 4 : 307 ~ 320
[학술지(정기간행물)] - Karasawa T / 2015 / An integrated view of cisplatin-induced nephrotoxicity and ototoxicity / Toxicol Lett 237 : 219 ~ 227
[학술지(정기간행물)] - Arany I / 2003 / Cisplatin nephrotoxicity / Semin Nephrol 23 : 460 ~ 464
[학술지(정기간행물)] - Pabla N / 2008 / Cisplatin nephrotoxicity : mechanisms and renoprotective strategies / Kidney Int 73 : 994 ~ 1007
[학술지(정기간행물)] - Wang D / 2005 / Cellular processing of platinum anticancer drugs / Nat Rev Drug Discov 4 : 307 ~ 320
[학술지(정기간행물)] - Karasawa T / 2015 / An integrated view of cisplatin-induced nephrotoxicity and ototoxicity / Toxicol Lett 237 : 219 ~ 227
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UCI(KEPA) : I410-ECN-0101-2018-524-001598916