지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 372 - 380 (9page)
이용수
초록· 키워드
오류제보하기
Mice with specific modified genes are useful means of studying development and disease. The CRISPR/Cas9 system is a very powerful and effective tool for generating genetically modified mice in a simple and fast manner. To generate human IgG light kappa constant knock-in mice, we tested by microinjection of a mixture of Cas9 protein, single-guide RNA and target homologous recombinant donor DNA into zygotes. We found that the injection of 10 ng/μL of Cas9 protein and crRNA/tracrRNA, rather than single guide RNA, induced the production of knock-in mice more effectively. Thus, our study provides valuable information that will help to improve the production of knock-in mice and contribute the successful generation of humanized Ab-producing mice in Korea.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (24)
참고문헌 신청
Aida T / 2016 / Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes / BMC Biotechnol 16:86~97
Beck A / 2010 / Strategies and challenges for the next generation of therapeutic antibodies / Nat Rev Immunol 10:345~352
Bibikova M / 2003 / Enhancing gene targeting with designed zinc finger nucleases / Science 300:764
Carlson DF / 2012 / Efficient TALEN-mediated gene knockout in livestock / Proc Natl Acad Sci U S A 109:1782~1787
Cho SW / 2013 / genome engineering in human cells with the Cas9 RNA-guided endonuclease / Nat Biotechnol 31:230~232
Beck A / 2010 / Strategies and challenges for the next generation of therapeutic antibodies / Nat Rev Immunol 10:345~352
Bibikova M / 2003 / Enhancing gene targeting with designed zinc finger nucleases / Science 300:764
Carlson DF / 2012 / Efficient TALEN-mediated gene knockout in livestock / Proc Natl Acad Sci U S A 109:1782~1787
Cho SW / 2013 / genome engineering in human cells with the Cas9 RNA-guided endonuclease / Nat Biotechnol 31:230~232
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Mouse model for genome editing therapeutics
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Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA
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UCI(KEPA) : I410-ECN-0101-2020-510-000238547