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논문 기본 정보

자료유형
학술저널
저자정보
Hyunbae Lee (Chungnam National University) Saminathan Subburaj (Chungnam National University) Luhua Tu (Chungnam National University) Ka-Yeon Lee (Chungnam National University) Gwangsu Park (Chungnam National University) Geung-Joo Lee (Chungnam National University)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.47 No.4
발행연도
2020.12
수록면
903 - 920 (18page)

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초록· 키워드

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Since its first demonstration as a practical genome editing tool in the early 2010s, the use of clustered regularly interspaced short palindromic repeat (CRISPR) along with the endonuclease Cas9 (CRISPR/Cas9) has become an essential choice for generating targeted mutations. Due to its relative simplicity and cost-effectiveness compared to other molecular scissors, i.e., zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), the CRISPR/Cas9 system has been shown to have a massive influence on genetic studies regardless of the biological kingdom. Although the system is in the process of being established, numerous protocols have already been released for the system and there have been various topics of CRISPR related papers published each year in ever-increasing manner. Here, we will briefly introduce CRISPR/Cas9 system and discuss the variants of the CRISPR system. Also, their applications to crop improvement will be dealt with mainly ornamental crops among horticultural crops other than Arabidopsis as a model plant. Finally, some issues on the barriers restraining the use of CRISPR system on floricultural crops, the prospect of CRISPR system as a DNA-free genome editing tool with efficient facilitators and finally, the future perspectives on the CRISPR system will be described.

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Abstract
Introduction
CRISPR/Cas9 variants
Application of CRISPR/Cas for floricultural crops
Factors impeding the application of CRISPR/Cas9 for ornamental species
DNA-free genome editing
Conclusion
References

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