지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.
목차
등록된 정보가 없습니다.
참고문헌 (104)
참고문헌 신청
Kosack CS / 2017 / A Guide to Aid the Selection of Diagnostic Tests / Bull. World Health Organ 95:639~645
Abudayyeh OO / 2016 / C2c2 Is a Single-Component Programmable RNA-Guided RNA-Targeting CRISPR Effector / Science (353)
East-Seletsky A / 2016 / Two Distinct RNase Activities of CRISPR-C2c2 Enable Guide-RNA Processing and RNA Detection / Nature 538:270~273
Ishino Y / 1987 / Nucleotide Sequence of the Iap Gene, Responsible for Alkaline Phosphatase Isoenzyme Conversion in Escherichia Coli, and Identification of the Gene Product / J. Bacteriol 169:5429~5433
Bolotin A / 2005 / Clustered Regularly Interspaced Short Palindrome Repeats(CRISPRs)Have Spacers of Extrachromosomal Origin / Microbiology 151:2551~2561
Abudayyeh OO / 2016 / C2c2 Is a Single-Component Programmable RNA-Guided RNA-Targeting CRISPR Effector / Science (353)
East-Seletsky A / 2016 / Two Distinct RNase Activities of CRISPR-C2c2 Enable Guide-RNA Processing and RNA Detection / Nature 538:270~273
Ishino Y / 1987 / Nucleotide Sequence of the Iap Gene, Responsible for Alkaline Phosphatase Isoenzyme Conversion in Escherichia Coli, and Identification of the Gene Product / J. Bacteriol 169:5429~5433
Bolotin A / 2005 / Clustered Regularly Interspaced Short Palindrome Repeats(CRISPRs)Have Spacers of Extrachromosomal Origin / Microbiology 151:2551~2561
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