지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 8 - 13 (6page)
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Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 µM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 µM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.
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등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Keith Pardee / 2016 / Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components / Cell 165(5):1255~1266
V. A. Brugman / 2018 / Detection of malaria sporozoites expelled during mosquito sugar feeding / Scientific Reports 8(1):7545
Fumihiko Kawamori / 2018 / Evaluation of Diagnostic Assay for Rickettsioses Using Duplex Real-Time PCR in Multiple Laboratories in Japan / Japanese Journal of Infectious Diseases 71(4):267~273
Kyung-Young Song / 2017 / Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications / Food Chemistry 229:341~346
Tae-Hoon Kim / 2017 / Development of a novel, rapid multiplex polymerase chain reaction assay for the detection and differentiation of Salmonella enterica serovars Enteritidis and Typhimurium using ultra-fast convection polymerase chain reaction / Foodborne Pathogens and Disease 14(10):580~586
V. A. Brugman / 2018 / Detection of malaria sporozoites expelled during mosquito sugar feeding / Scientific Reports 8(1):7545
Fumihiko Kawamori / 2018 / Evaluation of Diagnostic Assay for Rickettsioses Using Duplex Real-Time PCR in Multiple Laboratories in Japan / Japanese Journal of Infectious Diseases 71(4):267~273
Kyung-Young Song / 2017 / Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications / Food Chemistry 229:341~346
Tae-Hoon Kim / 2017 / Development of a novel, rapid multiplex polymerase chain reaction assay for the detection and differentiation of Salmonella enterica serovars Enteritidis and Typhimurium using ultra-fast convection polymerase chain reaction / Foodborne Pathogens and Disease 14(10):580~586
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