지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 1 - 9 (9page)
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초록· 키워드
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Background: Korean ginseng is an important cash crop in Asian countries. However, plant yield is reduced by pathogens. Among the Ilyonectria radicicola-species complex, I. mors-panacis is responsible for root-rot and replant failure of ginseng in Asia. The development of new methods to reveal the existence of the pathogen before cultivation is started is essential. Therefore, a quantitative real-time polymerase chain reaction method was developed to detect and quantify the pathogen in ginseng soils.
Methods: In this study, a species-specific histone H3 primer set was developed for the quantification of I. mors-panacis. The primer set was used on DNA from other microbes to evaluate its sensitivity and selectivity for I. mors-panacis DNA. Sterilized soil samples artificially infected with the pathogen at different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples.
Results: The designed primer set was found to be sensitive and selective for I. mors-panacis DNA. In artificially infected sterilized soil samples, using quantitative real-time polymerase chain reaction the estimated amount of template was positively correlated with the pathogen concentration in soil samples (R² =0.95), disease severity index (R² = 0.99), and colony-forming units (R² = 0.87). In natural soils, the pathogen was recorded in most fields producing bad yields at a range of 5.82±2.35 pg/g to 892.34 ± 103.70 pg/g of soil.
Conclusion: According to these results, the proposed primer set is applicable for estimating soil quality before ginseng cultivation. This will contribute to disease management and crop protection in the future.
Methods: In this study, a species-specific histone H3 primer set was developed for the quantification of I. mors-panacis. The primer set was used on DNA from other microbes to evaluate its sensitivity and selectivity for I. mors-panacis DNA. Sterilized soil samples artificially infected with the pathogen at different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples.
Results: The designed primer set was found to be sensitive and selective for I. mors-panacis DNA. In artificially infected sterilized soil samples, using quantitative real-time polymerase chain reaction the estimated amount of template was positively correlated with the pathogen concentration in soil samples (R² =0.95), disease severity index (R² = 0.99), and colony-forming units (R² = 0.87). In natural soils, the pathogen was recorded in most fields producing bad yields at a range of 5.82±2.35 pg/g to 892.34 ± 103.70 pg/g of soil.
Conclusion: According to these results, the proposed primer set is applicable for estimating soil quality before ginseng cultivation. This will contribute to disease management and crop protection in the future.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References
참고문헌 (29)
참고문헌 신청
김유진 / 2014 / Ginsenoside profiles and related gene expression during foliation in Panax ginseng Meyer / Journal of Ginseng Research 38(1):66~72
Leung KW / 2010 / Pharmacology of ginsenosides : a literature review / Chin Med 5:20
Fayeza Md Siraj / 2015 / Structural investigation of ginsenoside Rf with PPARg major transcriptional factor of adipogenesis and its impact on adipocyte / Journal of Ginseng Research 39(2):141~147
Ohh SH / 1992 / Studies on control of soil-borne diseases and insects of ginseng and development of antifungal compound. In: Korea Ginseng and Tobacco Research Inst / Ginseng Cultivation Bull :121~184
Yu YH / 1993 / Research on ginseng diseases in Korea / Korean J Ginseng Sci 17:61~68
Leung KW / 2010 / Pharmacology of ginsenosides : a literature review / Chin Med 5:20
Fayeza Md Siraj / 2015 / Structural investigation of ginsenoside Rf with PPARg major transcriptional factor of adipogenesis and its impact on adipocyte / Journal of Ginseng Research 39(2):141~147
Ohh SH / 1992 / Studies on control of soil-borne diseases and insects of ginseng and development of antifungal compound. In: Korea Ginseng and Tobacco Research Inst / Ginseng Cultivation Bull :121~184
Yu YH / 1993 / Research on ginseng diseases in Korea / Korean J Ginseng Sci 17:61~68
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UCI(KEPA) : I410-ECN-0101-2019-524-000425338