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논문 기본 정보

자료유형
학술저널
저자정보
Yan Huan huan (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. ChinaJiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal) Shang Yi tong (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. ChinaJiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal) Wang Li hong (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. ChinaJiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal) Tian Xue qin (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. ChinaJiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal) Tran Van-Tuan (VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam) Yao Li hua (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. China) Zeng Bin (Shenzhen Technology University, Shenzhen 518118, P.R. China) Hu Zhi hong (College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, P.R. ChinaJiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.5
발행연도
2024.5
수록면
1,178 - 1,187 (10page)
DOI
10.4014/jmb.2312.12003

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Cordyceps militaris is a significant edible fungus that produces a variety of bioactive compounds. We have previously established a uridine/uracil auxotrophic mutant and a corresponding Agrobacterium tumefaciens-mediated transformation (ATMT) system for genetic characterization in C. militaris using pyrG as a screening marker. In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic mutant of C. militaris. Using the uridine/uracil auxotrophic mutant as the background and pyrG as a selection marker, the hisB gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic C. militaris mutant. Then, pyrG in the histidine auxotrophic mutant was deleted to construct a ΔpyrG ΔhisB dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic C. militaris by using GFP and DsRed as reporter genes. Finally, to demonstrate the application of this dual transformation system for studies of gene function, knock out and complementation of the photoreceptor gene CmWC-1 in the dual auxotrophic C. militaris were performed. The newly constructed ATMT system with histidine and uridine/uracil auxotrophic markers provides a promising tool for genetic modifications in the medicinal fungus C. militaris.

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