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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 176 - 182 (7page)
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Background: Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR kit (InterLabService Ltd., Moscow, Russia). Methods: We used 159 samples from the lower respiratory tract (112 bronchoalveolar lavage [BAL] fluid, 37 sputum, and 10 endotracheal aspirate [ETA] samples) of non-HIV immunocompromised patients. Nested PCR and sequencing were used to resolve discordant results. The performance of the two assays was evaluated according to clinical categories (clinical Pneumocystis pneumonia [PCP], possible PCP, or unlikely PCP) based on clinical and radiological observations. Results: The positive and negative percent agreement values were 100% (95% confidence interval [CI], 85.4–100%) and 96.6% (95% CI, 90.9–98.9%), respectively, and kappa was 0.92 (95% CI, 0.84–0.99). P. jirovecii DNA load was significantly higher in the clinical PCP group than in the other groups (P<0.05). When stratified by sample type, the positive rate for BAL fluids from the clinical PCP group was 100% using either assay, whereas the positive rate for sputum/ETA samples was only 20%. Conclusions: The two assays showed similar diagnostic performance and detected low P. jirovecii burden in BAL fluids. Both assays may be useful as routine methods for detecting P. jirovecii DNA in a clinical laboratory setting, though their results should be interpreted considering sample type.
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등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Thomas CF Jr / 2004 / Pneumocystis pneumonia / N Engl J Med 350:2487~2498
White PL / 2017 / Diagnosis and management of Pneumocystis jirovecii infection / Expert Rev Anti Infect Ther 15:435~447
Alanio A / 2016 / ECIL guidelines for the diagnosis of Pneumocystis jirovecii pneumonia in patients with haematological malignancies and stem cell transplant recipients / J Antimicrob Chemother 71:2386~2396
Fauchier T / 2016 / Detection of Pneumocystis jirovecii by quantitative PCR to differentiate colonization and pneumonia in immunocompromised HIV-positive and HIV-negative patients / J Clin Microbiol 54:1487~1495
Robert-Gangneux F / 2014 / Diagnosis of Pneumocystis jirovecii pneumonia in immunocompromised patients by real-time PCR : a 4-year prospective study / J Clin Microbiol 52:3370~3376
White PL / 2017 / Diagnosis and management of Pneumocystis jirovecii infection / Expert Rev Anti Infect Ther 15:435~447
Alanio A / 2016 / ECIL guidelines for the diagnosis of Pneumocystis jirovecii pneumonia in patients with haematological malignancies and stem cell transplant recipients / J Antimicrob Chemother 71:2386~2396
Fauchier T / 2016 / Detection of Pneumocystis jirovecii by quantitative PCR to differentiate colonization and pneumonia in immunocompromised HIV-positive and HIV-negative patients / J Clin Microbiol 54:1487~1495
Robert-Gangneux F / 2014 / Diagnosis of Pneumocystis jirovecii pneumonia in immunocompromised patients by real-time PCR : a 4-year prospective study / J Clin Microbiol 52:3370~3376
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